Chulalongkorn University, Faculty of Graduate School, Interdisciplinary Program of Physiology, Bangkok, Thailand.
Chulalongkorn University, Faculty of Dentistry, Department of Physiology, Bangkok, Thailand.
J Appl Oral Sci. 2024 Nov 15;32:e20240251. doi: 10.1590/1678-7757-2024-0251. eCollection 2024.
Diabetes mellitus (DM) delays wound healing, including those following tooth extractions. Curcumin (CCM) can promote soft tissue and bone healing. The present study investigates the healing effects of CCM on tooth extraction sockets in diabetic rats.
Ninety-six male Wistar rats were divided into the following four groups: Control+Corn Oil (CO), Control+CCM, DM+CO, and DM+CCM. Each group was subdivided into 7-, 14-, and 28-day time point subgroups comprising eight rats. All animals had their maxillary first molars extracted. CCM-treated rats received 100 mg/kg of CCM orally for 7, 14, and 28 days. The lesion area was evaluated using macroscopic analyses, whereas socket healing was assessed by hematoxylin and eosin staining. Keratinocyte growth factor (KGF), Runt-related transcription factor 2 (Runx2), and collagen type I (COL1) expression levels were obtained using quantitative polymerase chain reaction (qPCR). Bone healing was analyzed by means of microcomputed tomography (μCT).
After 7 days, the groups showed no significant differences in lesion area and by day 14, no lesions were present. CCM treatment increased KGF mRNA expression in diabetic rats; however, diabetic rats showed delayed bone healing unrelated to CCM. CCM treatment resulted in increased Runx2 mRNA expression only in control rats, whereas COL1 mRNA expression remained unaffected by CCM.
CCM shows potential as a soft tissue healing enhancer in diabetic rats and could serve as an additional treatment to promote soft tissue repair in diabetic individuals. Although CCM did not impact alveolar bone healing, it may enhance bone healing in other skeleton regions.
糖尿病（DM）会延迟伤口愈合，包括拔牙后的愈合。姜黄素（CCM）可促进软组织和骨愈合。本研究探讨 CCM 对糖尿病大鼠拔牙窝愈合的影响。
96 只雄性 Wistar 大鼠分为以下四组：对照组+玉米油（CO）、对照组+CCM、糖尿病组+CO 和糖尿病组+CCM。每组再分为 7 天、14 天和 28 天时间点亚组，每组 8 只大鼠。所有动物均拔除上颌第一磨牙。CCM 治疗组大鼠连续 7、14 和 28 天口服 100mg/kg 的 CCM。使用大体分析评估病变面积，苏木精和伊红染色评估牙槽窝愈合。通过定量聚合酶链反应（qPCR）获得角质细胞生长因子（KGF）、 runt 相关转录因子 2（Runx2）和 I 型胶原蛋白（COL1）的表达水平。通过微计算机断层扫描（μCT）分析骨愈合情况。
第 7 天，各组间病变面积无显著差异，第 14 天无病变。CCM 治疗增加了糖尿病大鼠的 KGF mRNA 表达；然而，糖尿病大鼠的骨愈合延迟与 CCM 无关。CCM 治疗仅在对照组大鼠中增加了 Runx2 mRNA 表达，而 CCM 对 COL1 mRNA 表达没有影响。
CCM 可能成为糖尿病大鼠软组织愈合的增强剂，并可作为促进糖尿病患者软组织修复的附加治疗方法。尽管 CCM 对牙槽骨愈合没有影响，但它可能会增强其他骨骼区域的骨愈合。
