Harvey P R, Rupar C A, Gallinger S, Petrunka C N, Strasberg S M
Gut. 1986 Apr;27(4):374-81. doi: 10.1136/gut.27.4.374.
Human gall bladder mucus glycoprotein was isolated by Sepharose 4B gel filtration followed by caesium chloride density gradient ultracentrifugation from four groups: patients with cholesterol gall stones, patients with pigmented stones, patients with complete obstruction of the cystic duct and patients with no biliary tract abnormalities (controls). Mucus glycoprotein concentrations in cholesterol gall stone bile (203 micrograms/ml +/- 199 SD, n = 17), pigment gall stone bile (110 micrograms/ml +/- 77 SD, n = 6) and control gall bladder bile (96 micrograms/ml +/- 98 SD, n = 11) were not significantly different. While bile from patients with complete obstruction of the cystic duct contained significantly higher concentrations of mucus glycoprotein (6220 micrograms/ml +/- 4130, n = 4). In vitro cholesterol nucleation time was not correlated to gall bladder mucus glycoprotein concentrations. Qualitative analysis of the carbohydrate and amino acid composition showed a basic structure typical of mucus glycoproteins in general. It is unlikely that either quantitative or qualitative differences in mucus glycoproteins are responsible for the rapid in vitro nucleation time characteristic of cholesterol gall stone patients.
采用琼脂糖4B凝胶过滤法，随后进行氯化铯密度梯度超速离心，从四组人群中分离出人类胆囊黏液糖蛋白：胆固醇结石患者、色素结石患者、胆囊管完全梗阻患者以及无胆道异常患者（对照组）。胆固醇结石胆汁中的黏液糖蛋白浓度（203微克/毫升±199标准差，n = 17）、色素结石胆汁中的黏液糖蛋白浓度（110微克/毫升±77标准差，n = 6）与对照胆囊胆汁中的黏液糖蛋白浓度（96微克/毫升±98标准差，n = 11）无显著差异。而胆囊管完全梗阻患者的胆汁中黏液糖蛋白浓度显著更高（6220微克/毫升±4130，n = 4）。体外胆固醇成核时间与胆囊黏液糖蛋白浓度无关。对碳水化合物和氨基酸组成的定性分析显示，总体上具有黏液糖蛋白典型的基本结构。黏液糖蛋白在数量或质量上的差异不太可能是胆固醇结石患者体外快速成核时间的原因。
