Yui S, Karia K, Muzslay M, Wilson P, Ali S
Environmental Research Laboratory, University College London Hospital, London, UK.
Environmental Research Laboratory, University College London Hospital, London, UK.
J Hosp Infect. 2025 Jan;155:216-220. doi: 10.1016/j.jhin.2024.10.019. Epub 2024 Nov 19.
Ultraviolet C (UVC) decontamination systems are used in healthcare to supplement manual cleaning. Systems typically target whole rooms with fixed, vertical bulbs.
To evaluate the in-vitro and in-use efficacy of the novel device with emitters on articulating arms designed for rapid, targeted decontamination in a hospital room.
Isolates of meticillin-resistant Staphylococcus aureus (MRSA) (∼10 colony-forming units (cfu)), Klebsiella pneumoniae (∼10 cfu) and Clostridioides difficile spores (∼10 cfu) were inoculated on to stainless steel biological indicators (BIs) with low (0.3% BSA) and high (10% BSA/synthetic faeces) soiling. Bacteria were recovered from BIs following UVC decontamination, enumerated, and compared with controls. In-use efficacy was assessed by sampling aerobic colony counts (ACC) with contact plates. Sites were further sampled for C. difficile. Samples were taken before cleaning, after manual cleaning, and after UVC decontamination.
Reductions of 2.97-4.87 and 0.53-3.63 log cfu were demonstrated with MRSA and K. pneumoniae with low and high soiling, respectively. Efficacy against C. difficile was only observed in one location (1.12 log) with low soiling, but not synthetic faeces. ACC were highest on the bed foot rail (75 cfu/25 cm) and toilet flush (67 cfu/25 cm). Bacteria persisted on 50% of surfaces after manual cleaning and 30% after UVC decontamination. C. difficile persisted on one surface.
The system was effective for targeted disinfection and may be used for high-touch surfaces and equipment. The short cycle times allow operation in areas with minimal turnaround time such as operating theatres and anaesthetic rooms.
紫外线C（UVC）消毒系统用于医疗保健领域，以补充人工清洁。该系统通常使用固定的垂直灯泡对整个房间进行消毒。
评估一种新型设备的体外和实际使用效果，该设备带有用于医院病房快速、有针对性消毒的可旋转臂上的发射器。
将耐甲氧西林金黄色葡萄球菌（MRSA）（约10个菌落形成单位（cfu））、肺炎克雷伯菌（约10 cfu）和艰难梭菌孢子（约10 cfu）接种到低污染（0.3%牛血清白蛋白）和高污染（10%牛血清白蛋白/合成粪便）的不锈钢生物指示剂（BIs）上。在UVC消毒后从BIs中回收细菌，进行计数，并与对照组进行比较。通过使用接触平板对需氧菌落计数（ACC）进行采样来评估实际使用效果。还对艰难梭菌进行了进一步采样。在清洁前、人工清洁后和UVC消毒后采集样本。
对于低污染和高污染的MRSA和肺炎克雷伯菌，分别实现了2.97 - 4.87和0.53 - 3.63 log cfu的减少。仅在一个低污染位置观察到对艰难梭菌的消毒效果（1.12 log），但对合成粪便污染无效。ACC在床尾栏杆上最高（75 cfu/25 cm），马桶冲水处次之（67 cfu/25 cm）。人工清洁后50%的表面仍有细菌残留，UVC消毒后为30%。艰难梭菌在一个表面上持续存在。
该系统对于有针对性的消毒有效，可用于高接触表面和设备。短周期时间允许在周转时间最短的区域（如手术室和麻醉室）运行。
