Nowicki M J, Tong M J, Nair P V, Stevenson D
Hepatology. 1986 Mar-Apr;6(2):180-5. doi: 10.1002/hep.1840060205.
In vitro synthesis of the anti-HBc, anti-HBs and polyclonal IgG and IgM classes of antibodies were determined from supernatants of peripheral blood mono-nuclear cells cultured in the presence of pokeweed mitogen. Thirty-seven patients with chronic hepatitis B and 10 healthy control subjects whose sera were positive for anti-HBs formed the study group. Twenty-four of 37 patients showed histologic evidence of chronic active hepatitis B while the remaining 13 patients had chronic persistent hepatitis B. Lymphocytes from chronic persistent hepatitis B, chronic active hepatitis B and healthy controls secreted similar levels of IgM. However, IgG synthesis was markedly impaired (p less than 0.002) in the chronic persistent hepatitis B group as compared with healthy controls or chronic active hepatitis B patients. In vitro anti-HBc production and serum anti-HBc titers correlated directly with hepatocellular inflammation and inversely with serum hepatitis B virus DNA. Anti-HBc synthesis was significantly higher in chronic active hepatitis B patients who exhibited a more pronounced hepatocellular damage when compared to chronic persistent hepatitis B patients who had little or no liver cell injury.
在有商陆丝裂原存在的情况下，从培养的外周血单核细胞的上清液中测定抗 - HBc、抗 - HBs以及多克隆IgG和IgM类抗体的体外合成情况。37名慢性乙型肝炎患者和10名抗 - HBs血清阳性的健康对照者组成了研究组。37名患者中有24名显示出慢性活动性乙型肝炎的组织学证据，其余13名患者患有慢性持续性乙型肝炎。慢性持续性乙型肝炎、慢性活动性乙型肝炎患者及健康对照者的淋巴细胞分泌的IgM水平相似。然而，与健康对照者或慢性活动性乙型肝炎患者相比，慢性持续性乙型肝炎组的IgG合成明显受损（p小于0.002）。体外抗 - HBc产生及血清抗 - HBc滴度与肝细胞炎症直接相关，与血清乙型肝炎病毒DNA呈负相关。与几乎没有或没有肝细胞损伤的慢性持续性乙型肝炎患者相比，表现出更明显肝细胞损伤的慢性活动性乙型肝炎患者的抗 - HBc合成显著更高。
