Sheng Kun, Zhang Yang, Wu Chengzhi, Ni Jingshu, Huang Yao, Li Zhongsheng, Dong Meili, Wang Yikun, Liu Yong, Zhang Yuanzhi
Opt Express. 2024 Oct 7;32(21):36888-36906. doi: 10.1364/OE.537056.
We have proposed a novel single-snapshot spatial frequency domain imaging method with synchronous three-dimensional (3D) profile correction that addresses the confounding effects of involuntary jitter in tissue under examination and the 3D profile of the tissue on the measurements of optical parameters during in vivo examinations. I. In this scheme, orthogonal composite sinusoidal modulated light is projected onto the tissue to be measured. The single-snapshot multi-frequency demodulation theory, combined with the different sensitivity of different directional fringes to heights, simultaneously recovers the 3D profile and the modulated diffuse reflectance of the tissue to be measured. Finally, the modulated diffuse reflectance is corrected by establishing the correction function of height and angle versus modulated diffuse reflectance, and the optical parameters of the tissue are inverted by the diffusion model. The accuracy of the 3D profile measurement of the scheme was verified using models with different morphologies, and the mean value of the measurement error was found to be lower than 3%. The accuracy of modulation diffuse reflectance measurement was verified using a hemispherical homogeneous phantom made of PTFE. After correction with this method, the standard deviation of the measured diffuse reflectance is reduced by up to 72.2%. Finally, the measurement effect of optical parameters is verified by in vivo experiments and compared with the standard three-phase method. The results demonstrated that the 3D-SSMD method exhibits enhanced stability and efficiency. This introduces an innovative technology for achieving real-time spatial frequency domain imaging, applicable in clinical settings.
我们提出了一种新颖的单快照空间频域成像方法，该方法具有同步三维（3D）轮廓校正功能，可解决体内检查过程中被检查组织中不自主抖动的混杂效应以及组织的3D轮廓对光学参数测量的影响。一、在该方案中，将正交复合正弦调制光投射到待测组织上。单快照多频解调理论，结合不同方向条纹对高度的不同敏感度，同时恢复待测组织的3D轮廓和调制漫反射率。最后，通过建立高度和角度与调制漫反射率的校正函数来校正调制漫反射率，并利用扩散模型反演组织的光学参数。使用不同形态的模型验证了该方案3D轮廓测量的准确性，发现测量误差的平均值低于3%。使用由聚四氟乙烯制成的半球形均匀体模验证了调制漫反射率测量的准确性。用该方法校正后，测量的漫反射率的标准偏差降低了高达72.2%。最后，通过体内实验验证了光学参数的测量效果，并与标准三相法进行了比较。结果表明，3D-SSMD方法具有更高的稳定性和效率。这引入了一种用于实现实时空间频域成像的创新技术，适用于临床环境。
