Wang Ning, Lin Chenyi, Yang Zhongmin, Zhao Dan
College of Horticulture, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Front Plant Sci. 2024 Nov 7;15:1470780. doi: 10.3389/fpls.2024.1470780. eCollection 2024.
is an important plant germplasm resource, rich in nutrients and possessing unique medicinal value. However, due to its small floral organs, low seed setting rate of a single flower, high cost of artificial emasculation, and artificial pollination, the use of male sterile lines to prepare hybrids has become a common choice. In this study, var. Makino and were used as materials to study the regulation mechanism of anther development, aiming to provide a reference for male sterility. Through transcriptome differential gene screening and genome-wide bioinformatics analysis, 34 (S-phase kinase-associated protein 1) genes ( to ) were identified in the whole genome of The genes are unevenly distributed on eight chromosomes. Furthermore, two pairs of collinear relationships are evident among family members, and fragment replication events between genes have been identified. The phylogenetic tree analysis demonstrated that the , and genes were clustered into six groups, exhibiting a gene structure analogous to that observed in members of an evolutionary classification. A combination of gene structure and phylogenetic analysis revealed the presence of cis-acting elements associated with growth, hormone regulation, and stress response within the genes. Furthermore, expression analysis demonstrated that the genes exhibited differential expression patterns across various tissues of . The tissue-specific expression of the gene was verified by Real-Time PCR. A comparison of the two materials revealed significant differences in the expression of the gene in floral buds, the gene in inflorescence meristems, and the gene in inflorescence meristems, scapes, and floral buds. The results indicated that the three genes may be involved in anther development, thereby providing a theoretical basis for further study of floral organ development and pollen development in family members.
是一种重要的植物种质资源，营养丰富且具有独特的药用价值。然而，由于其花器官小、单花结实率低、人工去雄和人工授粉成本高，利用雄性不育系制备杂交种已成为常见选择。本研究以[品种名]Makino和[品种名]为材料，研究花药发育的调控机制，旨在为雄性不育研究提供参考。通过转录组差异基因筛选和全基因组生物信息学分析，在[植物名称]的全基因组中鉴定出34个S期激酶相关蛋白1（SKP1）基因（[基因编号区间]）。这些基因在8条染色体上分布不均。此外，家族成员之间存在两对共线关系，并且已鉴定出SKP1基因之间的片段复制事件。系统发育树分析表明，[具体基因名称1]、[具体基因名称2]和[具体基因名称3]基因聚为六组，呈现出与进化分类成员中观察到的类似基因结构。基因结构和系统发育分析相结合揭示了SKP1基因内存在与生长、激素调节和胁迫反应相关的顺式作用元件。此外，表达分析表明SKP1基因在[植物名称]的各个组织中表现出差异表达模式。通过实时荧光定量PCR验证了SKP1基因的组织特异性表达。两种材料的比较显示，花芽中[具体基因名称4]基因、花序分生组织中[具体基因名称5]基因以及花序分生组织、花茎和花芽中[具体基因名称6]基因的表达存在显著差异。结果表明这三个基因可能参与花药发育，从而为进一步研究[植物所属科名]家族成员的花器官发育和花粉发育提供了理论依据。
