Zhang Dongdong, Yu Zhenfan, Hu Shaobo, Liu Xingyue, Zeng Bin, Gao Wenwen, Qin HuanXue, Ma Xintong, He Yawen
College of Horticulture, Xinjiang Agriculture University, Urumqi, China.
GuangZhou Institute of Forestry and Landscape Architecture, GuangZhou, China.
Physiol Mol Biol Plants. 2023 Jan;29(1):35-49. doi: 10.1007/s12298-023-01278-9. Epub 2023 Jan 21.
(S-phase kinase-associated protein 1) is the core gene of SCF ubiquitin ligase, which mediates protein degradation, thereby regulating biological processes such as cell cycle progression, transcriptional regulation, and signal transduction. A variety of plant gene family studies have been reported. However, the almond gene family has not yet been studied. In this study, we identified 18 members of the family that were unevenly distributed across six chromosomes of the almond genome. Phylogenetic tree analysis revealed that the members can be divided into three groups: I, II, and III. members in each subfamily have relatively conserved motif types and exon/intron numbers. There were three pairs of fragment duplication genes and one pair of tandem repeat genes, and their functions were highly evolutionarily conserved. Transcriptome data showed that is expressed in almond flower tissues, and that its expression shows significant change during cross-pollination. Fluorescence quantitative results showed that eight genes had different expression levels in five tissues of almond, i.e., branches, leaves, flower buds, flesh, and cores. In addition, we cloned a gene based on . The cloned showed the same protein sequence as . Results of qPCR and western blot analysis showed high expression of in almond pollen. In conclusion, we report the first clone of the key gene that controls self-incompatibility in almonds. Our research lays a foundation for future functional research on members, especially for exploring the mechanism of almond self-incompatibility.
The online version contains supplementary material available at 10.1007/s12298-023-01278-9.
（S期激酶相关蛋白1）是SCF泛素连接酶的核心基因，其介导蛋白质降解，从而调节细胞周期进程、转录调控和信号转导等生物学过程。已经报道了多种植物基因家族研究。然而，杏仁基因家族尚未被研究。在本研究中，我们鉴定出该基因家族的18个成员，它们不均匀地分布在杏仁基因组的6条染色体上。系统发育树分析表明，这些成员可分为三组：I、II和III。每个亚家族中的成员具有相对保守的基序类型和外显子/内含子数量。有三对片段重复基因和一对串联重复基因，它们的功能在进化上高度保守。转录组数据表明，该基因在杏花组织中表达，并且其表达在异花授粉期间显示出显著变化。荧光定量结果表明，8个该基因在杏仁的五个组织（即枝条、叶片、花芽、果肉和果核）中具有不同的表达水平。此外，我们基于该基因克隆了一个基因。克隆的基因显示出与该基因相同的蛋白质序列。qPCR和蛋白质免疫印迹分析结果表明该基因在杏仁花粉中高表达。总之，我们报道了第一个克隆的控制杏仁自交不亲和性的关键基因。我们的研究为该基因家族成员的未来功能研究奠定了基础，特别是为探索杏仁自交不亲和性的机制奠定了基础。
在线版本包含可在10.1007/s12298-023-01278-9获取的补充材料。
