Chen Wei, Peng Xiayu, Kang Lichao, Dong Shengnan, Zhang Jian, Zhao Yunfeng, Sun Fengxia
School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China; Key Laboratory of Agricultural Product Processing and Quality Control of Specialty(Co-construction by Ministry and Province), School of Food Science and Technology, Shihezi University, Shihezi, China.
College of Animal Science and Technology, Shihezi University, Shihezi, China.
J Hazard Mater. 2025 Jan 15;482:136582. doi: 10.1016/j.jhazmat.2024.136582. Epub 2024 Nov 19.
The development of a versatile platform for bacterial assay and elimination is urgently needed due to the danger that bacteria pose to human life. Here, we synthesized a trimetallic deposition and horseradish peroxidase (HRP)-embedded porous coordination network-224 hybrid nanozymes (PCN-224 @AuPdPt@HRP) with outstanding peroxidase activity and fluorescence quenching ability. On this basis, we designed a dual recognition strategy-driven colorimetric-fluorescence dual-mode detection platform using Listeria monocytogenes as a pattern analyte. The platform consisted of an aptamer-modified PCN-224 @AuPdPt@HRP (PCN-224 @AuPdPt@HRP@Aptamer) specifically recognizing Listeria monocytogenes and vancomycin-coated 96-well plates. In the presence of vancomycin, which has the ability to recognize and inactivate gram-positive bacteria, the significant peroxidase activity of PCN-224 @AuPdPt@HRP@Aptamer in the precipitate was able to catalyze the color change of the substrate by HO. Meanwhile, the residual PCN-224 @AuPdPt@HRP@Aptamer in the supernatant was able to change the fluorescence of fluorescein-labeled deoxyribonucleic acid (FAM-DNA). In summary, this paper presents a multifunctional platform capable of detecting and eliminating residual bacteria in real environments. This strategy is expected to facilitate the development of multifunctional biosensors based on metal-organic framework probes and also provide environmental health.
由于细菌对人类生命构成的威胁，迫切需要开发一种用于细菌检测和消除的通用平台。在此，我们合成了一种具有出色过氧化物酶活性和荧光猝灭能力的三金属沉积和辣根过氧化物酶（HRP）嵌入的多孔配位网络-224杂化纳米酶（PCN-224@AuPdPt@HRP）。在此基础上，我们设计了一种以单核细胞增生李斯特菌为模式分析物的双识别策略驱动的比色-荧光双模式检测平台。该平台由特异性识别单核细胞增生李斯特菌的适配体修饰的PCN-224@AuPdPt@HRP（PCN-224@AuPdPt@HRP@Aptamer）和万古霉素包被的96孔板组成。在具有识别和灭活革兰氏阳性细菌能力的万古霉素存在下，沉淀物中PCN-224@AuPdPt@HRP@Aptamer的显著过氧化物酶活性能够催化底物被HO氧化而发生颜色变化。同时，上清液中残留的PCN-224@AuPdPt@HRP@Aptamer能够改变荧光素标记的脱氧核糖核酸（FAM-DNA）的荧光。综上所述，本文提出了一种能够检测和消除实际环境中残留细菌的多功能平台。该策略有望促进基于金属有机框架探针的多功能生物传感器的开发，并为环境卫生提供保障。
