Department of Obstetrics and Gynaecology, Faculty of Medicine Universitas Padjadjaran - Dr. Hasan Sadikin General Hospital, Pasteur No. 38, Bandung, West Java, 40161, Indonesia.
Bandung Fertility Center, Limijati Hospital, Bandung, Indonesia.
Sci Rep. 2024 Nov 22;14(1):29023. doi: 10.1038/s41598-024-71769-6.
Vitamin D and calcium in follicular fluid play an important role in modulating steroidogenesis, folliculogenesis, and oocyte quality determination. Both collaborate to produce top-quality embryos (TQE) during in vitro fertilization (IVF). In this study, we compared free 25(OH)D3 and calcium levels in follicular fluid between TQE and non-TQE groups. This cross-sectional study included women who underwent IVF procedures at tertiary hospitals in Bandung, Indonesia. Ovarian follicular fluid was collected during the ovum pick-up procedure. The examination of 25(OH)D3 levels, vitamin D-binding protein, and calcium in the follicles was done using an enzyme-linked immunosorbent assay (ELISA). Free 25(OH)D3 levels were calculated using the Vermeulen formula. A total of 173 samples met the study criteria, including 86 subjects in the TQE group and 87 subjects in the non-TQE group. There was a significant difference in free 25(OH)D3 follicular fluid levels between the TQE and non-TQE groups (p = 0.017); however, there was no significant difference in calcium levels between the two groups (p = 0.805). We also found that there was a significant association between free 25(OH)D3 follicular fluid levels and embryo quality (OR 3.05, 95% CI 1.46-6.38; p-value = 0.002); however, there was no significant association between follicular fluid calcium and embryo quality [p = 0.144 and OR, 1.74 (95% CI 0.82-3.68)]. The results suggest that free 25(OH)D3 and calcium in the follicular fluid act independently during steroidogenesis, folliculogenesis, and fertilization.
卵泡液中的维生素 D 和钙在调节类固醇生成、卵泡发生和卵母细胞质量决定中起着重要作用。两者共同作用，在体外受精 (IVF) 过程中产生优质胚胎 (TQE)。在这项研究中，我们比较了 TQE 和非 TQE 组卵泡液中的游离 25(OH)D3 和钙水平。这项横断面研究包括在印度尼西亚万隆的三级医院接受 IVF 程序的妇女。在取卵过程中收集卵巢卵泡液。使用酶联免疫吸附测定 (ELISA) 检查卵泡中的 25(OH)D3 水平、维生素 D 结合蛋白和钙。使用 Vermeulen 公式计算游离 25(OH)D3 水平。共有 173 个样本符合研究标准，包括 TQE 组 86 例和非 TQE 组 87 例。TQE 组和非 TQE 组卵泡液中游离 25(OH)D3 水平有显著差异（p=0.017）；然而，两组间钙水平无显著差异（p=0.805）。我们还发现，卵泡液中游离 25(OH)D3 水平与胚胎质量之间存在显著关联（OR 3.05，95%CI 1.46-6.38；p 值=0.002）；然而，卵泡液钙与胚胎质量之间无显著关联 [p=0.144 和 OR，1.74（95%CI 0.82-3.68)]。结果表明，卵泡液中的游离 25(OH)D3 和钙在类固醇生成、卵泡发生和受精过程中独立作用。
