Jones C L, Khan S A
J Bacteriol. 1986 Apr;166(1):29-33. doi: 10.1128/jb.166.1.29-33.1986.
The complete nucleotide sequence of the enterotoxin B gene from Staphylococcus aureus, as well as the 5' and 3' flanking regions, was determined. Starting from an ATG initiator codon, an open reading frame encoded the enterotoxin B precursor that consisted of 266 amino acids (Mr, 31,400). The 5' terminal portion of the gene encodes a signal peptide 27 amino acids long. The deduced amino acid sequence matched, with a few exceptions, the published amino acid sequence of enterotoxin B. The structural gene was flanked on the 5' side by a promoter-like sequence and on the 3' side by a palindromic structure followed by a thymine-rich region that resembled a transcription termination signal. Downstream from the entB gene were two overlapping open reading frames corresponding to 134 and 185 amino acids in the opposite orientation. The signal sequence of the enterotoxin B precursor resembled that of other secreted proteins found in other bacteria.
测定了金黄色葡萄球菌肠毒素B基因以及5'和3'侧翼区域的完整核苷酸序列。从ATG起始密码子开始，一个开放阅读框编码了由266个氨基酸组成的肠毒素B前体（Mr，31,400）。该基因的5'末端部分编码一个27个氨基酸长的信号肽。推导的氨基酸序列与已发表的肠毒素B氨基酸序列基本匹配，仅有少数例外。结构基因在5'侧由一个类似启动子的序列侧翼，在3'侧由一个回文结构侧翼，随后是一个富含胸腺嘧啶的区域，类似于转录终止信号。entB基因下游有两个重叠的开放阅读框，方向相反，分别对应134和185个氨基酸。肠毒素B前体的信号序列与其他细菌中发现的其他分泌蛋白的信号序列相似。
