Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China.
College of Biology and Food Engineering, Anyang Institute of Technology, Anyang, China.
Pest Manag Sci. 2019 Oct;75(10):2744-2755. doi: 10.1002/ps.5384. Epub 2019 Mar 21.
Many insect cytochrome P450 proteins (CYPs) are involved in the metabolic detoxification of exogenous compounds such as plant toxins and insecticides. Tribolium castaneum, the red flour beetle, is a major agricultural pest that damages stored grains and cereal products. With the completion of the sequencing of its genome, two T. castaneum species-specific CYP genes, CYP4BN6, and CYP6BQ11, were identified. However, it is unknown whether the functions of most CYPs are shared by TcCYP4BN6 and TcCYP6BQ11, and the upstream regulatory mechanism of these two CYPs remains elusive.
QRT-PCR analysis indicated that TcCYP4BN6 and TcCYP6BQ11 were both most highly expressed at the late pupal stage and were mainly observed in the head and gut, respectively, of adults. Moreover, the transcripts of these two CYPs were significantly induced by dichlorvos and carbofuran, and RNA interference (RNAi) targeting of each of them enhanced the susceptibility of beetles to these two insecticides. Intriguingly, knockdown of the latrophilin (lph) gene, which has been reported to be related to the insecticide susceptibility, reduced the expression of TcCYP4BN6 and TcCYP6BQ11 after insecticide treatment, suggesting that these two CYP genes are regulated by lph to participate in insecticide susceptibility in T. castaneum.
These results shed new light on the function and mechanism of CYP genes associated with insecticide susceptibility and could facilitate research on appropriate and sustainable pest control management. © 2019 Society of Chemical Industry.
许多昆虫细胞色素 P450 蛋白（CYPs）参与外来化合物如植物毒素和杀虫剂的代谢解毒。赤拟谷盗，即红麴虫，是一种主要的农业害虫，会损害储存的谷物和谷类产品。随着其基因组测序的完成，鉴定出了两个赤拟谷盗种特异性 CYP 基因，CYP4BN6 和 CYP6BQ11。然而，尚不清楚 TcCYP4BN6 和 TcCYP6BQ11 是否具有大多数 CYP 的功能，并且这两个 CYP 的上游调控机制仍不清楚。
实时荧光定量 PCR 分析表明，TcCYP4BN6 和 TcCYP6BQ11 在后期蛹期表达量最高，分别主要在成虫的头部和肠道中表达。此外，这两个 CYP 的转录物均被二氯松和呋喃丹显著诱导，而针对它们各自的 RNA 干扰（RNAi）增强了甲虫对这两种杀虫剂的敏感性。有趣的是，已报道与杀虫剂敏感性相关的拉普罗林（lph）基因的敲低降低了杀虫剂处理后 TcCYP4BN6 和 TcCYP6BQ11 的表达，表明这两个 CYP 基因受 lph 调控参与赤拟谷盗对杀虫剂的敏感性。
这些结果为与杀虫剂敏感性相关的 CYP 基因的功能和机制提供了新的认识，并有助于研究适当和可持续的害虫管理。 © 2019 化学工业协会。
