Ali Ramadan, Alshaman Reem, Albalawi Abdullah S, Alsharif Osama M, Hakami Osama A, Alharthi Hamzah Hussain, Jumah Faisal F, Al-Qarni Abdulrhman M, Albalawi Naif S, Alashjaee Meshari Saud, Aljohani Faisal S, Alghuzawi Abdulaziz M, Al-Atwi Muath H, Alharbi Abdullah H, El-Wekil Mohamed M
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi Arabia.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi Arabia.
Food Chem. 2025 Feb 15;465(Pt 2):142076. doi: 10.1016/j.foodchem.2024.142076. Epub 2024 Nov 16.
Terbium and nitrogen co-doped carbon dots (Tb@N-CDs), combined with α-lipoic acid-functionalized copper nanoclusters (LA@CuNCs), were proposed for the ratiometric detection of quinolone (QA) antibiotics. In this system, Tb@N-CDs facilitate the aggregation of LA@CuNCs, enhancing its fluorescence emission at 670 nm via aggregation-induced emission enhancement (AIEE). Meanwhile, the fluorescence emission of Tb@N-CDs at 460 nm diminishes due to Förster resonance energy transfer (FRET). Upon the introduction of QA, the binding between Tb ions and N-CDs weakens, disrupting both AIEE and FRET processes. This disruption results in a reduction in fluorescence emission at 670 nm and a concurrent increase at 460 nm. The fluorescence response ratio (F/F) increases with higher concentrations of ciprofloxacin (CFX), demonstrating a linear range from 0.008 to 120 μmol L and LOD of 1.6 nmol L. The method successfully detected CFX in milk and urine samples, achieving recoveries between 97.7 % and 103.8 %, RSD of less than 3.79 %.
铽氮共掺杂碳点（Tb@N-CDs）与α-硫辛酸功能化铜纳米簇（LA@CuNCs）相结合，用于喹诺酮（QA）类抗生素的比率检测。在该体系中，Tb@N-CDs促进LA@CuNCs的聚集，通过聚集诱导发光增强（AIEE）增强其在670nm处的荧光发射。同时，由于Förster共振能量转移（FRET），Tb@N-CDs在460nm处的荧光发射减弱。引入QA后，Tb离子与N-CDs之间的结合减弱，破坏了AIEE和FRET过程。这种破坏导致670nm处的荧光发射减少，同时460nm处的荧光发射增加。荧光响应比率（F/F）随环丙沙星（CFX）浓度的升高而增加，线性范围为0.008至120μmol·L，检出限为1.6nmol·L。该方法成功检测了牛奶和尿液样品中的CFX，回收率在97.7%至103.8%之间，相对标准偏差小于3.79%。
