Zhao Xinming, Yu Jialuo, Lin Dong, Han Limei, Li Bowei, Chen Jiadong, Wu Yixuan, Huang Yan, Chen Lingxin, Wang Xiaoyan
School of Pharmacy, Binzhou Medical University, Yantai, 264003, China.
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Research Centre for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
Talanta. 2025 May 1;286:127537. doi: 10.1016/j.talanta.2025.127537. Epub 2025 Jan 4.
Ciprofloxacin (CIP) is a commonly used antibiotic, but its abuse may cause bacterial resistance, posing a high risk to the environment and human health. Herein, based on the molecular imprinting technology, this study proposed a ratiometric fluorescence sensor employing the "post-doping" strategy, which aims to be rapid, selective, and visually easy-to-use for CIP detection to address antibiotic residues and environmental risks. Specifically, by exploiting the "antenna effect" of lanthanide metal ions (Ln), terbium (III) (Tb) chosen as a fluorescence-assisted functional monomer as well as the red emitting CdTe quantum dots (QDs) as the internal reference signal were introduced into multi-emission Tb-CdTe@SiO@MIPs (TbMIPs). With the increase of CIP concentration, the variations among the fluorescence peaks within a multi-emission ratiometric fluorescence sensor not only induce a broader range of color changes, but also produce a more sensitive and stable effect through self-internal standard correction. The sensor showed a good trend in a wide concentration range within 0.08-50 μM and provided a satisfactory detection limit of 0.018 μM, which had lower detection limit for CIP than most of methods. It had demonstrated exceptional selectivity and had been successfully used to efficiently detect CIP in real samples including seawater, river water, milk and urine. Furthermore, when integrated with smartphone-based data processing capabilities, this sensor enabled highly sensitive visual detection under the 365 nm UV lamp device. Through innovative development, the wide color transition observed with the sensor makes it highly suitable for practical applications, enhancing its usability for further real-time monitoring.
环丙沙星（CIP）是一种常用抗生素，但其滥用可能导致细菌耐药性，对环境和人类健康构成高风险。在此，基于分子印迹技术，本研究提出了一种采用“后掺杂”策略的比率荧光传感器，旨在实现对CIP检测的快速、选择性和视觉上易于使用，以解决抗生素残留和环境风险问题。具体而言，通过利用镧系金属离子（Ln）的“天线效应”，选择铽（III）（Tb）作为荧光辅助功能单体，并将红色发射的碲化镉量子点（QDs）作为内部参考信号引入到多发射Tb-CdTe@SiO@MIPs（TbMIPs）中。随着CIP浓度的增加，多发射比率荧光传感器内荧光峰之间的变化不仅会引起更广泛的颜色变化，还会通过自内标校正产生更灵敏和稳定的效果。该传感器在0.08 - 50 μM的宽浓度范围内表现出良好的趋势，提供了令人满意的0.018 μM检测限，对CIP的检测限低于大多数方法。它表现出优异的选择性，并已成功用于高效检测包括海水、河水、牛奶和尿液在内的实际样品中的CIP。此外，当与基于智能手机的数据处理能力集成时，该传感器在365 nm紫外灯装置下实现了高灵敏度的视觉检测。通过创新发展，该传感器观察到的宽颜色转变使其非常适合实际应用，增强了其用于进一步实时监测的实用性。
