Ren Minyi, Li Meng, Boccaccini Aldo R, Xu Yan, Li Lu, Zheng Kai
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing 210029, China; Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing 210029, China; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing 210029, China.
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstrasse 6, Erlangen 91058, Germany.
Int J Biol Macromol. 2025 Jan;284(Pt 1):137954. doi: 10.1016/j.ijbiomac.2024.137954. Epub 2024 Nov 22.
Periodontal tissue defects are a leading cause of tooth loss in adults. Guided tissue regeneration (GTR) has emerged as an effective clinical approach for promoting periodontal regeneration. In this study, we employed a green electrospinning technology to combine recombinant human-like collagen (RHC) with polycaprolactone (PCL) to fabricate RHC/PCL membranes for periodontal regeneration. Our results showed that incorporating RHC into PCL up to 40 wt% did not significantly affect the nanofibrous structure of the PCL membranes. When the RHC concentration exceeded 10 wt%, both the tensile strength and modulus of the PCL membranes increased. Additionally, the presence of RHC improved the hydrophilicity of membranes. Biologically, the addition of RHC, regardless of its concentration, significantly enhanced the adhesion, proliferation, and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs). These effects were confirmed through various assays, including the CCK-8 assay, live/dead staining, as well as ALP and ARS staining. Further, real-time quantitative PCR and Western blot analysis revealed upregulation of osteogenesis-related genes and proteins. Transcriptome sequencing analysis suggested that the PI3K-Akt signaling pathway might play a critical role in the enhanced osteogenesis induced by RHC. Our results emphasize the potential of RHC/PCL nanofibrous membranes to advance the field of periodontal tissue repair.
牙周组织缺损是成年人牙齿缺失的主要原因。引导组织再生（GTR）已成为促进牙周再生的一种有效临床方法。在本研究中，我们采用绿色静电纺丝技术将重组类人胶原蛋白（RHC）与聚己内酯（PCL）相结合，制备用于牙周再生的RHC/PCL膜。我们的结果表明，将RHC以高达40 wt%的比例掺入PCL中，对PCL膜的纳米纤维结构没有显著影响。当RHC浓度超过10 wt%时，PCL膜的拉伸强度和模量均增加。此外，RHC的存在改善了膜的亲水性。从生物学角度来看，无论RHC浓度如何，其添加均显著增强了人牙周膜干细胞（hPDLSCs）的黏附、增殖和成骨分化。这些效应通过各种检测得以证实，包括CCK-8检测、活/死染色以及碱性磷酸酶（ALP）和茜素红染色（ARS）。此外，实时定量PCR和蛋白质印迹分析显示成骨相关基因和蛋白质上调。转录组测序分析表明，PI3K-Akt信号通路可能在RHC诱导的增强成骨过程中起关键作用。我们的结果强调了RHC/PCL纳米纤维膜在推进牙周组织修复领域的潜力。
