Zhong Pingxiang, Chen Pengyun, Huo Pengju, Ma Lei, Xu Zhaoxian, Li Fuguang, Cai Chenggu
Zhengzhou Research Base, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450000, China; Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
College of Resources and Environmental Sciences, National Academy of Agriculture Green Development, Key Laboratory of Plant-Soil Interactions, Ministry of Education, China Agricultural University, Beijing 100193, China.
Bioresour Technol. 2025 Feb;417:131797. doi: 10.1016/j.biortech.2024.131797. Epub 2024 Nov 22.
Cotton stalk, an important by-product of cotton farming, is challenging in lignocellulosic feedstock application due to the limited understanding of their compositional and lignin structural characteristics. This study elucidates the composition of lignocellulose components and fundamental lignin structural features of cotton stalk. Lignocellulosic hydrolysates were prepared from various cotton stalk parts and used for single-cell protein production. As a proof of concept, cotton stalk hydrolysates were successfully converted into single-cell protein using the superior microbial host, Candida utilis ACCC20060, owing to its favorable sugar consumption efficiency and high protein quality. The highest SCP concentration of 5.74 g/L was obtained, yielding 0.23 g/g from the lignocellulose-derived sugars released from cotton stalk roots. This study provides valuable references for cotton stalk utilization toward lignocellulosic feedstock application and introduces a promising microbial host for single-cell protein production from such feedstocks.
棉秆是棉花种植的一种重要副产品，由于对其组成和木质素结构特征的了解有限，在木质纤维素原料应用方面具有挑战性。本研究阐明了棉秆木质纤维素成分的组成和基本木质素结构特征。从棉秆的不同部位制备了木质纤维素水解产物，并用于单细胞蛋白的生产。作为概念验证，由于其良好的糖消耗效率和高蛋白质量，使用优良的微生物宿主产朊假丝酵母ACCC20060成功地将棉秆水解产物转化为单细胞蛋白。获得了最高5.74 g/L的单细胞蛋白浓度，从棉秆根部释放的木质纤维素衍生糖中产生了0.23 g/g。本研究为棉秆在木质纤维素原料应用中的利用提供了有价值的参考，并介绍了一种用于从此类原料生产单细胞蛋白的有前景的微生物宿主。
