Bellil Zahra, Meyer Sylvain, Tilloy Valentin, Mairi Assia, De Champs Christophe, Barraud Olivier, Touati Abdelaziz
Université de Bejaia, Laboratoire d'Ecologie Microbienne, FSNV, Algérie.
Université de Limoges, INSERM, CHU Limoges, RESINFIT, U1092, Limoges, France.
Foodborne Pathog Dis. 2024 Nov 25. doi: 10.1089/fpd.2024.0036.
Nontyphoidal presents a significant threat to animal and human health as a food-borne infectious agent. This study focused on the characterization of isolates obtained from fresh animal products consumed in Bejaia, Algeria. In total, 495 beef products, 600 chicken products, and 355 dairy products were collected from retail outlets and slaughterhouses in the region. Out of the 1450 samples collected, a positivity rate of 3.5% (51/1450) was observed. Traditional Algerian sausages exhibited the highest prevalence of contamination (14.1%, 36/256). Fifteen different serotypes were identified, with Kentucky ( = 12), Anatum ( = 11), and Bredeney ( = 9) being the most prevalent. Antimicrobial susceptibility testing showed resistance to diverse antibiotics, particularly against tetracycline, ampicillin, nalidixic acid, and sulphonamides. Whole-genome sequencing conducted on 21 isolates enabled the comparison of phylogenetic links between isolates. We notably identified clones circulating across the region in different locations and food types, suggesting contamination at the early stages of the food chain (in the herd or slaughterhouses) that disseminated to numerous butcher shops in various cities. We also identified acquired antibiotic resistance genes and point mutations in the quinolone resistance-determining region genes, contributing to the observed resistance patterns. This study describes the genomic and phylogenetic characteristics of several clones found in diverse food samples in Algeria. It suggests potential transmission dynamics that could better understand 's contamination routes.
非伤寒型[细菌名称未给出]作为一种食源性感染因子，对动物和人类健康构成重大威胁。本研究聚焦于从阿尔及利亚贝贾亚消费的新鲜动物产品中分离出的[细菌名称未给出]菌株的特征分析。总共从该地区的零售店和屠宰场收集了495份牛肉产品、600份鸡肉产品和355份乳制品。在收集的1450个样本中，观察到阳性率为3.5%（51/1450）。传统的阿尔及利亚香肠显示出[细菌名称未给出]污染的最高流行率（14.1%，36/256）。鉴定出了15种不同的血清型，其中肯塔基（=12）、阿纳托姆（=11）和布雷德尼（=9）最为常见。抗菌药敏试验表明对多种抗生素耐药，尤其是对四环素、氨苄青霉素、萘啶酸和磺胺类药物。对21株分离株进行的全基因组测序能够比较分离株之间的系统发育联系。我们特别鉴定出在该地区不同地点和食物类型中传播的克隆，这表明在食物链的早期阶段（在畜群或屠宰场）受到污染，并传播到各个城市的众多肉店。我们还鉴定出获得性抗生素耐药基因以及喹诺酮耐药决定区基因中的点突变，这导致了观察到的耐药模式。本研究描述了在阿尔及利亚不同食物样本中发现的几种[细菌名称未给出]克隆的基因组和系统发育特征。它表明了潜在的传播动态，有助于更好地理解[细菌名称未给出]的污染途径。
