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KS-cSCC-1和KS-cSCC-2：从日本患者中建立的两种新型皮肤鳞状细胞癌细胞系。

KS-cSCC-1 and KS-cSCC-2: two novel cutaneous squamous cell carcinoma cell lines established from Japanese patients.

作者信息

Ito Takamichi, Tanaka Yuka, Kaku-Ito Yumiko, Tanegashima Keiko, Imajima Mao, Ichiki Toshio, Nakahara Takeshi

机构信息

Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

出版信息

Front Med (Lausanne). 2024 Nov 7;11:1483450. doi: 10.3389/fmed.2024.1483450. eCollection 2024.

DOI:10.3389/fmed.2024.1483450
PMID:39582977
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11583063/
Abstract

INTRODUCTION

Cutaneous squamous cell carcinoma (cSCC) is a common form of skin cancer. Less accessibility to the cSCC cell lines has limited analyses of this disease. Thus, we here aimed to establish novel cSCC cell lines from patient's cSCC lesions.

METHODS

Two novel cSCC cell lines (named KS-cSCC-1 and KS-cSCC-2) were established from an axillary lymph node metastasis of a Japanese female and an inguinal lymph node metastasis of a Japanese male. The characteristics of the established cell lines were assessed by analyses.

RESULTS

The cells were successfully maintained for more than 9 months, with a doubling time of 47.5 ± 1.11 h (KS-cSCC-1) and 39.2 ± 5.78 h (KS-cSCC-2). The cell lines exhibited constant growth, spheroid formation, and invasiveness. Short tandem repeat analyses and immunohistochemistry confirmed that both cell lines are identical to their original tumor. The KS-cSCC-1 cells were weakly positive for CK14 and strongly positive for CK10, while the KS-cSCC-2 showed opposite expression patterns. Chemosensitivity of the cell lines was further tested and the cells were sensitive to anticancer drugs which are used to treat cSCC.

CONCLUSION

The KS-cSCC-1 and KS-cSCC-2 cell lines were promising resources for basic and preclinical research on cSCC to better define the tumor characteristics and treatment strategy of this cancer.

摘要

引言

皮肤鳞状细胞癌（cSCC）是一种常见的皮肤癌形式。获取cSCC细胞系的难度较大，限制了对该疾病的分析。因此，我们旨在从患者的cSCC病变中建立新的cSCC细胞系。

方法

从一名日本女性的腋窝淋巴结转移灶和一名日本男性的腹股沟淋巴结转移灶中建立了两个新的cSCC细胞系（分别命名为KS-cSCC-1和KS-cSCC-2）。通过分析评估所建立细胞系的特征。

结果

这些细胞成功维持了9个月以上，倍增时间分别为47.5±1.11小时（KS-cSCC-1）和39.2±5.78小时（KS-cSCC-2）。这些细胞系表现出持续生长、球体形成和侵袭性。短串联重复序列分析和免疫组织化学证实，这两个细胞系与其原始肿瘤相同。KS-cSCC-1细胞对CK14呈弱阳性，对CK10呈强阳性，而KS-cSCC-2表现出相反的表达模式。进一步测试了细胞系的化学敏感性，这些细胞对用于治疗cSCC的抗癌药物敏感。

结论

KS-cSCC-1和KS-cSCC-2细胞系是用于cSCC基础和临床前研究的有前景的资源，有助于更好地明确该癌症的肿瘤特征和治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39ca/11583063/e0bd00897899/fmed-11-1483450-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39ca/11583063/5aa9da32b7d5/fmed-11-1483450-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39ca/11583063/71fed3c7a8ba/fmed-11-1483450-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39ca/11583063/376624f0530f/fmed-11-1483450-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39ca/11583063/e68b4d94b935/fmed-11-1483450-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39ca/11583063/e0bd00897899/fmed-11-1483450-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39ca/11583063/5aa9da32b7d5/fmed-11-1483450-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39ca/11583063/71fed3c7a8ba/fmed-11-1483450-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39ca/11583063/376624f0530f/fmed-11-1483450-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39ca/11583063/e68b4d94b935/fmed-11-1483450-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39ca/11583063/e0bd00897899/fmed-11-1483450-g0005.jpg

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