Crittenden Sarah L, Dos Santos Stephany J Costa, Battula Sindhu, Kimble Judith
Department of Biochemistry, University of Wisconsin-Madison.
Present: WiCell Research Institute Madison, WI.
MicroPubl Biol. 2024 Nov 7;2024. doi: 10.17912/micropub.biology.001391. eCollection 2024.
The distal tip cell niche uses GLP-1 /Notch signaling to maintain germline stem cells. The RAM domain, which resides within the intracellular portion of the GLP-1 /Notch receptor, is integral to formation of a signaling-dependent transcription activation complex. Here we report the generation of a mutation in the GLP-1 RAM domain, created in a GLP-1 /Notch receptor with a C-terminal V5 tag. The phenotype of homozygotes is similar to that of null mutants, but expression of the GLP-1 (RAM ) protein was normal in the heterozygotes. We conclude that the RAM mutation abolishes receptor activity.
远端末梢细胞生态位利用GLP-1/Notch信号通路来维持生殖系干细胞。RAM结构域位于GLP-1/Notch受体的细胞内部分，对于信号依赖的转录激活复合物的形成不可或缺。在此，我们报告了在带有C端V5标签的GLP-1/Notch受体中产生的GLP-1 RAM结构域突变。纯合子的表型与无效突变体相似，但杂合子中GLP-1（RAM ）蛋白的表达正常。我们得出结论，RAM突变消除了受体活性。
