一种体细胞蛋白聚糖控制Notch介导的生殖细胞命运。

A somatic proteoglycan controls Notch-directed germ cell fate.

作者信息

Gopal Sandeep, Amran Aqilah, Elton Andre, Ng Leelee, Pocock Roger

机构信息

Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Victoria, 3800, Australia.

出版信息

Nat Commun. 2021 Nov 18;12(1):6708. doi: 10.1038/s41467-021-27039-4.

Communication between the soma and germline optimizes germ cell fate programs. Notch receptors are key determinants of germ cell fate but how somatic signals direct Notch-dependent germ cell behavior is undefined. Here we demonstrate that SDN-1 (syndecan-1), a somatic transmembrane proteoglycan, controls expression of the GLP-1 (germline proliferation-1) Notch receptor in the Caenorhabditis elegans germline. We find that SDN-1 control of a somatic TRP calcium channel governs calcium-dependent binding of an AP-2 transcription factor (APTF-2) to the glp-1 promoter. Hence, SDN-1 signaling promotes GLP-1 expression and mitotic germ cell fate. Together, these data reveal SDN-1 as a putative communication nexus between the germline and its somatic environment to control germ cell fate decisions.

体细胞与生殖系之间的通讯优化了生殖细胞命运程序。Notch受体是生殖细胞命运的关键决定因素，但体细胞信号如何指导Notch依赖性生殖细胞行为尚不清楚。在这里，我们证明了SDN-1（syndecan-1），一种体细胞跨膜蛋白聚糖，控制秀丽隐杆线虫生殖系中GLP-1（生殖系增殖-1）Notch受体的表达。我们发现，SDN-1对体细胞TRP钙通道的控制决定了AP-2转录因子（APTF-2）与glp-1启动子的钙依赖性结合。因此，SDN-1信号促进GLP-1表达和有丝分裂生殖细胞命运。这些数据共同揭示了SDN-1是生殖系与其体细胞环境之间控制生殖细胞命运决定的一个假定通讯枢纽。