Park J H, Grandjean C J, Antonson D L, Vanderhoof J A
J Nutr. 1986 Apr;116(4):610-7. doi: 10.1093/jn/116.4.610.
We investigated the effects of zinc deficiency on body composition by using intragastric force-feeding to obviate decreased food intake and altered eating patterns. Weanling male Sprague-Dawley rats were fed a purified zinc-deficient diet: the ad libitum-fed control group (AL; eight rats) was given powdered diet and water containing 25 ppm zinc; the zinc-replete group (ZN; nine rats) was force-fed a diet blended with water containing zinc in an amount of equal caloric intake to the AL group and allowed access to water containing zinc. The zinc intake of ZN rats was approximately twice that of AL rats based on water intake. The zinc-deficient group (ZD; 13 rats) was fed similarly to the ZN group except deionized water was used for diet preparation and drinking water. After 8 d, body and muscle weight were lower in the ZD group than in the ZN group. Femur weights were similar in the two groups. Serum, liver and femur zinc concentrations were 85, 22 and 42% lower, respectively, in the ZD group than in the ZN group. Serum glucose, relative liver weight, liver glycogen and liver lipids were higher, but muscle and liver DNA were lower in the ZD group than in control groups.
我们通过采用灌胃强制喂食法来避免食物摄入量减少和饮食模式改变，从而研究锌缺乏对身体成分的影响。将断乳雄性Sprague-Dawley大鼠喂食纯化的缺锌饮食：自由采食对照组（AL组，8只大鼠）给予含25 ppm锌的粉状饮食和水；锌充足组（ZN组，9只大鼠）被强制喂食与含锌水混合的饮食，其热量摄入量与AL组相等，并可饮用含锌水。基于饮水量，ZN组大鼠的锌摄入量约为AL组的两倍。缺锌组（ZD组，13只大鼠）的喂养方式与ZN组相似，只是在制备饮食和饮用水时使用去离子水。8天后，ZD组的体重和肌肉重量低于ZN组。两组的股骨重量相似。ZD组的血清、肝脏和股骨锌浓度分别比ZN组低85%、22%和42%。ZD组的血清葡萄糖、相对肝脏重量、肝糖原和肝脂质较高，但肌肉和肝脏DNA低于对照组。
