Park J H, Grandjean C J, Hart M H, Erdman S H, Pour P, Vanderhoof J A
Am J Physiol. 1986 Sep;251(3 Pt 1):E273-8. doi: 10.1152/ajpendo.1986.251.3.E273.
The effect of zinc deficiency on glucose tolerance was investigated using intragastric force feeding to obviate decreased food intake and altered eating patterns. Three groups of weanling male Sprague-Dawley rats were fed a purified zinc-deficient diet: zinc-deficient, ad libitum-fed animals (ZDA) were offered powdered zinc-deficient diet; zinc-replete, force-fed controls (ZRF) were tube fed a diet blended with water containing 25 ppm of zinc; zinc-deficient, force-fed animals (ZDF) were similarly tube fed the zinc-deficient diet. The ZRF and ZDF groups received a diet of identical amount based on the intake of ad libitum-fed, zinc-replete rats. After 8 days of feeding, the ZDF group had impaired glucose tolerance curves, yet blood insulin and glucagon levels were normal. The ZDA group had normal glucose tolerance with low insulin levels compared with the ZRF group. The islet cell morphology among the three dietary groups were similar. These results suggest that the glucose intolerance observed in ZDF rats is not due to altered blood insulin and glucagon levels but rather to peripheral resistance to insulin action.
采用灌胃强制喂食法以避免食物摄入量减少和进食模式改变，研究了锌缺乏对葡萄糖耐量的影响。将三组断乳雄性斯普拉格-道利大鼠喂食纯化的缺锌饮食：缺锌自由采食动物（ZDA）喂食缺锌粉状饮食；补锌强制喂食对照（ZRF）通过胃管喂食与含25 ppm锌的水混合的饮食；缺锌强制喂食动物（ZDF）同样通过胃管喂食缺锌饮食。ZRF组和ZDF组根据自由采食、补锌大鼠的摄入量给予相同量的饮食。喂食8天后，ZDF组的葡萄糖耐量曲线受损，但血液胰岛素和胰高血糖素水平正常。与ZRF组相比，ZDA组葡萄糖耐量正常但胰岛素水平较低。三个饮食组的胰岛细胞形态相似。这些结果表明，ZDF大鼠中观察到的葡萄糖不耐受不是由于血液胰岛素和胰高血糖素水平改变，而是由于外周对胰岛素作用的抵抗。
