基于 RNA 测序的大肠杆菌感染奶牛乳腺炎相关潜在关键环状 RNA 的鉴定：初步研究结果。

Identification of potential key circular RNAs associated with Escherichia coli-infected bovine mastitis using RNA-sequencing: preliminary study results.

机构信息

College of Animal Science and Technology, Ningxia University, Yinchuan, 750021, Ningxia Hui Autonomous Region, People's Republic of China.

Key Laboratory of Ruminant Molecular Cell Breeding, Yinchuan, 750021, Ningxia Hui Autonomous Region, People's Republic of China.

出版信息

Vet Res Commun. 2024 Nov 26;49(1):36. doi: 10.1007/s11259-024-10592-w.

DOI:10.1007/s11259-024-10592-w

PMID:39589641

Abstract

Escherichia coli (E. coli) is commonly found in dairy farms and can invade mammary gland tissue, often causing acute clinical mastitis. Mammary infections with E. coli have shown a wide range of clinical signs, causing abnormal appearance of the milk, udder inflammation and systemic signs of illness. Circular RNA is a class of endogenous non-coding RNA that plays an important role in the occurrence and development of various inflammatory diseases. However, there is little information on the circRNA associated with bovine mastitis. In this study investigated the involvement of circRNAs in bovine mastitis through the construction of an E. coli-infected bovine mastitis model by injecting of E. coli into the mammary gland of dairy cows, using healthy gland mammary tissue as a control (M_C). High-throughput RNA-seq was performed on the E. coli-infected mammary gland tissue (M_E) and differentially expressed circRNAs between theM_C and M_E groups, followed by an analysis of their potential functions using bioinformatics methods. A total of 164 differentially expressed circRNAs were identified, including 92 downregulated circRNAs and 72 upregulated circRNAs. As shown by Gene Ontology enrichment analysis these DE circRNAs were mostly enriched in ras protein signal transduction, cytoplasmic vesicle parts, and enzyme binding, and Kyoto Encyclopedia of Genes and genome singal pathway enrichment analysis indicated significant associations with phagosome signal pathway. Additionally, the expression of bovine mastitis-related circRNAs, including novel_circRNA_0000128, novel_circRNA_0011103, novel_circRNA_0012656, novel_circRNA_0015099, novel_circRNA_005648, novel_circRNA_000074, and novel_circRNA_0011796 were verified via quantitative reverse-transcription polymerase chain reaction (RT-qPCR). These results provide a new direction for further investigation of the molecular mechanisms underlying bovine mastitis.

摘要

大肠杆菌（E. coli）通常存在于奶牛场，可以入侵乳腺组织，常引起急性临床乳腺炎。大肠杆菌引起的乳腺炎表现出广泛的临床症状，导致牛奶外观异常、乳房炎症和全身疾病症状。环状 RNA 是一类内源性非编码 RNA，在各种炎症性疾病的发生和发展中发挥重要作用。然而，关于与牛乳腺炎相关的 circRNA 的信息很少。在这项研究中，通过向奶牛乳腺注射大肠杆菌构建大肠杆菌感染牛乳腺炎模型，以健康乳腺组织为对照（M_C），研究 circRNA 在牛乳腺炎中的作用。对大肠杆菌感染的乳腺组织（M_E）进行高通量 RNA-seq 分析，筛选出差异表达的 circRNAs，并用生物信息学方法分析其潜在功能。共鉴定出 164 个差异表达的 circRNAs，包括 92 个下调的 circRNAs 和 72 个上调的 circRNAs。GO 富集分析表明，这些 DE circRNAs 主要富集在 Ras 蛋白信号转导、细胞质小泡部分和酶结合等功能，KEGG 通路富集分析表明与吞噬体信号通路显著相关。此外，通过定量逆转录聚合酶链反应（RT-qPCR）验证了与牛乳腺炎相关的 circRNAs，包括 novel_circRNA_0000128、novel_circRNA_0011103、novel_circRNA_0012656、novel_circRNA_0015099、novel_circRNA_005648、novel_circRNA_000074 和 novel_circRNA_0011796 的表达。这些结果为进一步研究牛乳腺炎的分子机制提供了新的方向。