Virus-Based Neural Circuit Tracing.

Neural circuits provide an anatomical basis for functional networks. Therefore, dissecting the structure of neural circuits is an indispensable prerequisite to understanding how the brain functions. Knowing how the neural circuits organize and function under physiological conditions and their progressive alterations under pathophysiological conditions are key to understanding the underlying circuit mechanism of diseases, thus finding cures for the diseases. Recombinant neurotropic viruses are important tools for neural circuit tracing with many advantages over non-viral tracers: they allow for anterograde, retrograde, and trans-synaptic delivery of tracers in a cell-type-specific, circuit-selective manner. We herein summarize the recent developments in the viral tools for neural circuit tracing, discuss the key principles of using viral tools in neuroscience research, and highlight innovations for developing and optimizing viral tools for neural circuit tracing across diverse animal species, including nonhuman primates.