Choi Minji, Kwon Jiyoung, Jang Ji-Hyun, Kim Duck-Su, Kim Hyun-Jung
Medical Science Research Institute, Kyung Hee University Medical Center, Seoul 02447, Republic of Korea.
Department of Conservative Dentistry, Kyung Hee University Dental Hospital, Seoul 02447, Republic of Korea.
J Funct Biomater. 2024 Nov 10;15(11):337. doi: 10.3390/jfb15110337.
(1) Background: This study aimed to enhance the biological properties of hydraulic calcium silicate cements (HCSCs) by incorporating organic and inorganic components, specifically elastin-like polypeptides (ELPs) and bioactive glass (BAG). We focused on the effects of these composites on the viability, migration, and osteogenic differentiation of human periodontal ligament fibroblasts (hPDLFs). (2) Methods: Proroot MTA was supplemented with 1-5 wt% 63S BAG and 10 wt% ELP. The experimental groups contained various combinations of HSCS with ELP and BAG. Cell viability was assessed using an MTT assay, cell migration was evaluated using wound healing and transwell assays, and osteogenic activity was determined through Alizarin Red S staining and a gene expression analysis of osteogenic markers (, , , and ). (3) Results: The combination of ELP and BAG significantly enhanced the viability of hPDLFs with an optimal BAG concentration of 1-4%. Cell migration assays demonstrated faster migration rates in groups with 2-4% BAG and ELP incorporation. Osteogenic activity was the highest with 2-3% BAG incorporation with ELP, as evidenced by intense Alizarin Red S staining and the upregulation of osteogenic differentiation markers. (4) Conclusions: The incorporation of ELP (organic) and BAG (inorganic) into HCSC significantly enhances the viability, migration, and osteogenic differentiation of hPDLFs. These findings suggest that composite HCSC might support healing in destructed bone lesions in endodontics.
(1) 背景：本研究旨在通过掺入有机和无机成分，特别是弹性蛋白样多肽（ELP）和生物活性玻璃（BAG）来增强水硬性硅酸钙水泥（HCSC）的生物学性能。我们重点研究了这些复合材料对人牙周膜成纤维细胞（hPDLFs）的活力、迁移和成骨分化的影响。(2) 方法：在Proroot MTA中添加1 - 5 wt%的63S BAG和10 wt%的ELP。实验组包含HCSC与ELP和BAG的各种组合。使用MTT法评估细胞活力，通过伤口愈合和Transwell实验评估细胞迁移，并通过茜素红S染色和对成骨标志物（、、和）的基因表达分析来确定成骨活性。(3) 结果：ELP和BAG的组合显著提高了hPDLFs的活力，最佳BAG浓度为1 - 4%。细胞迁移实验表明，在掺入2 - 4% BAG和ELP的组中迁移速率更快。茜素红S染色强烈以及成骨分化标志物上调表明，掺入2 - 3% BAG与ELP时成骨活性最高。(4) 结论：将ELP（有机）和BAG（无机）掺入HCSC可显著提高hPDLFs的活力、迁移和成骨分化。这些发现表明复合HCSC可能有助于牙髓病学中受损骨病变的愈合。
