Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, Poland.
Mass Spectrometry Centre, LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Santiago University Campus, 3810-193 Aveiro, Portugal.
Mar Drugs. 2024 Nov 10;22(11):509. doi: 10.3390/md22110509.
Lipid extracts from the microalgae and have great potential to prevent ultraviolet A (UVA)-induced metabolic disorders. Therefore, the aim of this study has been to analyze their cytoprotective effect, focused on maintaining intracellular redox balance and inflammation in UVA-irradiated skin fibroblasts, at the proteome level. The above lipid extracts reversed the suppression of the antioxidant response caused by UVA radiation, which was more visible in the case of . Modulations of interactions between heme oxygenase-1 and matrix metalloproteinase 1/Parkinson's disease protein 7/transcript1-α/β, as well as thioredoxin and migration inhibitory factor/Parkinson's disease protein 7/calnexin/ATPase p97, created key molecular signaling underlying their cytoprotective actions. Moreover, they reduced pro-inflammatory processes in the control group but they also showed the potential to regulate the cellular inflammatory response by changing inflammasome signaling associated with the changes in the caspase-1 interaction area, including heat shock proteins HSP90, HSPA8, and vimentin. Therefore, lipid extracts from and protect skin fibroblast metabolism from UVA-induced damage by restoring the redox balance and regulating inflammatory signaling pathways. Thus, those extracts have proven to have great potential to be used in cosmetic or cosmeceutical products to protect the skin against the effects of solar radiation. However, the possibility of their use requires the evaluation of their effects at the skin level in in vivo and clinical studies.
从微藻 和 中提取的脂质具有预防紫外线 A（UVA）诱导的代谢紊乱的巨大潜力。因此，本研究旨在从蛋白质组水平分析其细胞保护作用，重点是维持 UVA 照射皮肤成纤维细胞的细胞内氧化还原平衡和炎症。上述脂质提取物逆转了 UVA 辐射引起的抗氧化反应抑制， 在 中更为明显。血红素加氧酶-1 与基质金属蛋白酶 1/帕金森病蛋白 7/转录 1-α/β 以及硫氧还蛋白与迁移抑制因子/帕金森病蛋白 7/钙联蛋白/ATP 酶 p97 之间相互作用的调节，为其细胞保护作用的关键分子信号通路创造了条件。此外，它们减少了对照组中的促炎过程，但它们还具有通过改变与半胱天冬酶-1 相互作用区域变化相关的炎症小体信号来调节细胞炎症反应的潜力，包括热休克蛋白 HSP90、HSPA8 和波形蛋白。因此， 和 从 UVA 诱导的损伤中保护皮肤成纤维细胞代谢，通过恢复氧化还原平衡和调节炎症信号通路来实现。因此，这些提取物已被证明具有很大的潜力，可用于化妆品或美容产品中，以保护皮肤免受太阳辐射的影响。然而，它们的使用可能性需要在体内和临床研究中评估其对皮肤的影响。
