Gęgotek Agnieszka, Conde Tiago, Domingues Maria Rosário, Domingues Pedro, Skrzydlewska Elżbieta
Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, Poland.
Centre for Environmental and Marine Studies, Department of Chemistry, University of Aveiro, Santiago University Campus, 3810-193 Aveiro, Portugal.
Cells. 2024 Nov 21;13(23):1934. doi: 10.3390/cells13231934.
Melanoma is one of the most malignant forms of skin cancer, characterised by the highest mortality rate among affected patients. This study aims to analyse and compare the effects of lipid extracts from the microalgae () and () on the intra and extracellular proteome of UVA-irradiated melanoma cells using a three-dimensional model. Proteomic analysis revealed that UVA radiation significantly increases the levels of pro-inflammatory proteins in melanoma cells. Treatment with algae extracts reduced these protein levels in both non-irradiated and irradiated cells. Furthermore, untreated cells released proteins responsible for cell growth and proliferation into the medium, a process hindered by UVA radiation through the promotion of pro-inflammatory molecules secretion. The treatment with algae extracts effectively mitigated UVA-induced alterations. Notably, UVA radiation significantly induced the formation of 4-HNE and 15-PGJ2 protein adducts in both cells and the medium, while treatment with algae extracts stimulated the formation of 4-HNE-protein adducts and reduced the level of 15-PGJ2-protein adducts. However, both algae extracts successfully prevented these UVA-induced modifications. In conclusion, lipid extracts from and appear to be promising agents in supporting anti-melanoma therapy. However, their potent protective capacity may limit their applicability, particularly following cells exposure to UVA.
黑色素瘤是皮肤癌最恶性的形式之一，其特征是在受影响患者中死亡率最高。本研究旨在使用三维模型分析和比较微藻（）和（）的脂质提取物对紫外线A（UVA）照射的黑色素瘤细胞细胞内和细胞外蛋白质组的影响。蛋白质组学分析表明，UVA辐射显著增加黑色素瘤细胞中促炎蛋白的水平。用藻类提取物处理可降低未照射和照射细胞中的这些蛋白质水平。此外，未处理的细胞将负责细胞生长和增殖的蛋白质释放到培养基中，这一过程受到UVA辐射的阻碍，因为UVA辐射会促进促炎分子的分泌。用藻类提取物处理可有效减轻UVA诱导的改变。值得注意的是，UVA辐射在细胞和培养基中均显著诱导4-羟基壬烯醛（4-HNE）和15-脱氧-Δ12,14-前列腺素J2（15-PGJ2）蛋白加合物的形成，而用藻类提取物处理则刺激4-HNE蛋白加合物的形成并降低15-PGJ2蛋白加合物的水平。然而，两种藻类提取物均成功阻止了这些UVA诱导的修饰。总之，（）和（）的脂质提取物似乎是支持抗黑色素瘤治疗的有前景的药物。然而，它们强大的保护能力可能会限制其适用性，尤其是在细胞暴露于UVA之后。
