UCD School of Biosystems and Food Engineering, University College Dublin, Belfield, 4 Dublin, Ireland.
Teagasc Ashtown Food Research Centre, Teagasc, 15 Dublin, Ireland.
Mar Drugs. 2024 Nov 14;22(11):516. doi: 10.3390/md22110516.
The solid phase byproduct obtained after conventional fucoidan extraction from the brown seaweed can be used as a source containing alginate. This study involves ultrasound-assisted extraction (UAE) of alginate from the byproduct using sodium bicarbonate. Response surface methodology (RSM) was applied to obtain the optimum conditions for alginate extraction. The ultrasound (US) treatments included 20 kHz of frequency, 20-91% of amplitude, and an extraction time of 6-34 min. The studied investigated the crude alginate yield (%), molecular weight, and alginate content (%) of the extracts. The optimum conditions for obtaining alginate with low molecular weight were found to be 69% US amplitude and sonication time of 30 min. The alginate extracts obtained were characterized using Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). Ultrasound-assisted extraction involving a short treatment lasting 6-34 min was found to be effective in extracting alginate from the byproduct compared to the conventional extraction of alginate using stirring at 415 rpm and 60 °C for 24 h. The US treatments did not adversely impact the alginate obtained, and the extracted alginates were found to have similar characteristics to the alginate obtained from conventional extraction and commercial sodium alginate.
从褐藻中常规提取岩藻聚糖后的固相副产物可作为含有褐藻胶的来源。本研究采用碳酸氢钠从副产物中超声辅助提取（UAE）褐藻胶。响应面法（RSM）用于获得褐藻胶提取的最佳条件。超声（US）处理包括 20 kHz 的频率、20-91%的振幅和 6-34 分钟的提取时间。研究了粗褐藻胶产率（%）、分子量和提取物中褐藻胶含量（%）。获得低分子量褐藻胶的最佳条件为 69%的 US 振幅和 30 分钟的超声时间。用傅里叶变换红外（FTIR）光谱、热重分析（TGA）和差示扫描量热法（DSC）对褐藻胶提取物进行了表征。与在 415 rpm 和 60°C 下搅拌 24 小时的常规提取相比，涉及持续 6-34 分钟的短处理时间的超声辅助提取在从副产物中提取褐藻胶方面更为有效。US 处理对获得的褐藻胶没有不利影响，并且提取的褐藻胶与从常规提取和商业褐藻酸钠获得的褐藻胶具有相似的特性。
