Kazakov Petar, Alseekh Saleh, Ivanova Valentina, Gechev Tsanko
Center of Plant Systems Biology and Biotechnology, 14 Knyaz Boris I Pokrastitel Str., 4023 Plovdiv, Bulgaria.
Max Planck Institute of Molecular Plant Physiology, 1 Am Muehlenberg, 14476 Potsdam, Germany.
Metabolites. 2024 Nov 5;14(11):594. doi: 10.3390/metabo14110594.
BACKGROUND/OBJECTIVES: The biostimulant SuperFifty, produced from the brown algae , can improve crop quality and yield and mitigate stress tolerance in model and crop plants such as , pepper, and tomato. However, the effect of SuperFifty on raspberries and strawberries has not been well studied, especially in terms of nutritional properties and yield. The aim of this study was to investigate the effect of SuperFifty on the quality and quantity of raspberry and strawberry fruits, with a focus on metabolic composition and essential elements, which together determine the nutritional properties and total yield of these two crops.
Metabolome analysis was performed by liquid chromatography-mass spectrometry analysis (LC-MS), and essential elements analysis was performed by inductively coupled plasma-mass spectrometry (ICP-MS).
Here, we demonstrate that SuperFifty increases the fruit size of both raspberries and strawberries and enhances the yield in these two berry crops by 42.1% (raspberry) and 33.9% (strawberry) while preserving the nutritional properties of the fruits. Metabolome analysis of 100 metabolites revealed that antioxidants, essential amino acids, organic acids, sugars, and vitamins, such as glutathione, alanine, asparagine, histidine, threonine, serine, tryptophan, sucrose, citric acid, pantothenic acid (vitamin B5), as well as other primary metabolites, remain the same in the SuperFifty-primed fruits. Secondary metabolites, such as caffeic acid, p-coumaric acid, kaempferol, and quercetin, also maintained their levels in the SuperFifty-primed fruits. Analysis of essential elements demonstrated that elements important for human health, such as Zn, Mn, Fe, B, Cu, K, and Ca, maintain the same levels in the raspberry and strawberry fruits obtained from the biostimulant-primed plants. Magnesium, an important element known as a co-factor in many enzymatic reactions related to both plant physiology and human health, increased in both raspberry and strawberry fruits primed with SuperFifty. Finally, we discuss the potential financial and health benefits of the SuperFifty-induced priming for both growers and consumers.
We demonstrate that SuperFifty significantly enhances the yield of both raspberries and strawberries, improves the marketable grade of the fruits (larger and heavier fruits), and enhances the nutritional properties by elevating Mg content in the fruits. Altogether, this biostimulant-induced molecular priming offers an environmentally friendly, efficient, and sustainable way to enhance the yield and quality of berry crops, with clear benefits to both berry producers and customers.
背景/目的：由褐藻制成的生物刺激剂SuperFifty可提高作物品质和产量，并减轻模式植物和作物（如辣椒和番茄）的胁迫耐受性。然而，SuperFifty对树莓和草莓的影响尚未得到充分研究，尤其是在营养特性和产量方面。本研究的目的是调查SuperFifty对树莓和草莓果实品质和产量的影响，重点关注代谢成分和必需元素，这些共同决定了这两种作物的营养特性和总产量。
通过液相色谱-质谱分析（LC-MS）进行代谢组分析，并通过电感耦合等离子体质谱（ICP-MS）进行必需元素分析。
在此，我们证明SuperFifty增加了树莓和草莓的果实大小，并使这两种浆果作物的产量分别提高了42.1%（树莓）和33.9%（草莓），同时保持了果实的营养特性。对100种代谢物的代谢组分析表明，抗氧化剂、必需氨基酸、有机酸、糖和维生素，如谷胱甘肽、丙氨酸、天冬酰胺、组氨酸、苏氨酸、丝氨酸、色氨酸、蔗糖、柠檬酸、泛酸（维生素B5）以及其他初级代谢物，在经SuperFifty处理的果实中保持不变。次生代谢物，如咖啡酸、对香豆酸、山奈酚和槲皮素，在经SuperFifty处理过的果实中也保持其含量水平。必需元素分析表明，对人体健康重要的元素，如锌、锰、铁、硼、铜、钾和钙，在从经生物刺激剂处理的植物中获得的树莓和草莓果实中保持相同水平。镁是一种重要元素，在许多与植物生理和人体健康相关的酶促反应中作为辅助因子，在经SuperFifty处理的树莓和草莓果实中均有所增加。最后，我们讨论了SuperFifty诱导处理对种植者和消费者的潜在经济和健康益处。
我们证明SuperFifty显著提高了树莓和草莓的产量，改善了果实的可销售等级（果实更大、更重），并通过提高果实中的镁含量增强了营养特性。总之，这种生物刺激剂诱导的分子预处理为提高浆果作物的产量和品质提供了一种环境友好、高效且可持续的方法，对浆果生产者和消费者都有明显益处。
