Song Jing, Wang Hongqiong, Li Shizhu, Qian Peijun, Wang Wenya, Shen Meifen, Zhang Zongya, Zhou Jihua, Li Chunying, Yang Zaogai, Hao Yuwan, Du Chunhong, Dong Yi
Department of Schistosomiasis Control and Prevention, Yunnan Institute of Endemic Disease Control and Prevention, Dali, People's Republic of China.
Yunnan Key Laboratory of Natural Focus Disease Control Technology, Dali, People's Republic of China.
PLoS Negl Trop Dis. 2024 Nov 26;18(11):e0012094. doi: 10.1371/journal.pntd.0012094. eCollection 2024 Nov.
Oncomelania hupensis robertsoni is the only intermediate host of Schistosoma japonicum in hilly regions of south-west China, which plays a key role during the transmission of Schistosomiasis. This study aimed to sequence the complete mitochondrial genome of Oncomelania hupensis robertsoni and analyze genetic differentiation of Oncomelania hupensis robertsoni.
Samples were from 13 villages in Yunnan Province of China, with 30 Oncomelania hupensis snails per village, and the complete mitochondrial genome was sequenced. A comprehensive analysis of the genetic differentiation of Oncomelania hupensis robertsoni was conducted by constructing phylogenetic trees, calculating genetic distances, and analyzing identity.
A total of 26 complete mitochondrial sequences were determined. The length of genome ranged from 15,181 to 15,187 bp, and the base composition of the genome was A+T (67.5%) and G+C content (32.5%). This genome encoded 37 genes, including 13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes. The phylogenetic trees and identity analysis confirmed that Oncomelania hupensis robertsoni was subdivided into Oncomelania hupensis robertsoni Yunnan strain and Sichuan strain, with a genetic distance of 0.0834. Oncomelania hupensis robertsoni Yunnan strain was further subdivided into two sub-branches, corresponding to "Yunnan North" and "Yunnan South", with a genetic distance of 0.0216, and the samples exhibited over 97% identity.
Oncomelania hupensis robertsoni is subdivided into Oncomelania hupensis robertsoni Yunnan strain and Sichuan strain. Oncomelania hupensis robertsoni Yunnan strain exhibits a higher level of genetic identity and clear north-south differentiation. This work reported the first mitochondrial genome of Oncomelania hupensis robertsoni Yunnan strain, which could be used as an important reference genome for Oncomelania hupensis, and also provide the important information for explaining the distribution pattern of Oncomelania hupensis robertsoni and control of Schistosoma japonicum.
云南钉螺是中国西南山区日本血吸虫唯一的中间宿主，在血吸虫病传播中起关键作用。本研究旨在对云南钉螺的线粒体基因组进行测序，并分析云南钉螺的遗传分化情况。
样本来自中国云南省的13个村庄，每个村庄采集30只云南钉螺，对其线粒体基因组进行测序。通过构建系统发育树、计算遗传距离和分析同一性，对云南钉螺的遗传分化进行综合分析。
共测定了26条完整的线粒体序列。基因组长度在15181至15187 bp之间，基因组的碱基组成为A+T（67.5%），G+C含量为（32.5%）。该基因组编码37个基因，包括13个蛋白质编码基因、2个rRNA基因和22个tRNA基因。系统发育树和同一性分析证实，云南钉螺分为云南株和四川株，遗传距离为0.0834。云南钉螺云南株进一步分为两个亚分支，分别对应“云南北部”和“云南南部”，遗传距离为0.0216，样本间同一性超过97%。
云南钉螺分为云南株和四川株。云南钉螺云南株表现出较高的遗传同一性和明显的南北分化。本研究首次报道了云南钉螺云南株的线粒体基因组，可为云南钉螺提供重要的参考基因组，也为解释云南钉螺的分布格局和日本血吸虫的防控提供重要信息。
