细胞培养新手指南：预防不必要问题的实用建议。

A Beginner's Guide to Cell Culture: Practical Advice for Preventing Needless Problems.

机构信息

Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, D-52074 Aachen, Germany.

Electron Microscopy Facility, Institute of Pathology, RWTH University Hospital Aachen, D-52074 Aachen, Germany.

出版信息

Cells. 2023 Feb 21;12(5):682. doi: 10.3390/cells12050682.

The cultivation of cells in a favorable artificial environment has become a versatile tool in cellular and molecular biology. Cultured primary cells and continuous cell lines are indispensable in investigations of basic, biomedical, and translation research. However, despite their important role, cell lines are frequently misidentified or contaminated by other cells, bacteria, fungi, yeast, viruses, or chemicals. In addition, handling and manipulating of cells is associated with specific biological and chemical hazards requiring special safeguards such as biosafety cabinets, enclosed containers, and other specialized protective equipment to minimize the risk of exposure to hazardous materials and to guarantee aseptic work conditions. This review provides a brief introduction about the most common problems encountered in cell culture laboratories and some guidelines on preventing or tackling respective problems.

在有利的人工环境中培养细胞已成为细胞和分子生物学中一种通用的工具。原代细胞和连续细胞系在基础、生物医学和转化研究的研究中是不可或缺的。然而，尽管它们具有重要的作用，但细胞系经常被其他细胞、细菌、真菌、酵母、病毒或化学物质所误识别或污染。此外，细胞的处理和操作与特定的生物和化学危害有关，需要特殊的保护措施，如生物安全柜、封闭容器和其他专用防护设备，以最大程度地降低接触危险物质的风险，并保证无菌工作条件。本文简要介绍了细胞培养实验室中最常见的问题，并就预防或解决相应问题提供了一些指导原则。