ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) Research Group, Faculty of Dentistry, Complutense University, 28040 Madrid, Spain.
Department of Conservative Dentistry, Faculty of Dentistry, University of Chile, Santiago 8380544, Chile.
Cells. 2024 Nov 13;13(22):1877. doi: 10.3390/cells13221877.
The aim of this in vitro study was to evaluate the effect of xanthohumol and thymol on the impact of on the structure, size and cell viability of subgingival biofilms formed on dental implant surfaces. The structure and microbial biomass of biofilms developed after 72 h, treated and untreated with both extracts, were compared by scanning electron microscopy (SEM) and confocal laser microscopy (CLSM). Quantitative polymerase chain reaction (qPCR) was used to quantify the number of viable and total microorganisms of each of the biofilm-forming strains in each condition. A general linear model was used to compare and validate the CLSM and qPCR results. The presence of xanthohumol and thymol during biofilm development inhibited the filamentous growth of . The biofilm incubated with xanthohumol had significantly lower bacterial biomass and cell viability than the biofilm not exposed to the extract ( < 0.05). In contrast, these global parameters showed no differences when the biofilm was incubated with thymol. In the presence of xanthohumol, there was a decrease in counts and cell viability of , , and . Thymol treatment reduced the viability of and . The presence of these vegetable extracts during the development of a dynamic in vitro multispecies biofilm model inhibited the filamentous growth of , partially reversing the effect that the fungus exerted on the structure, size and vitality of periodontopathogenic bacteria.
本体外研究旨在评估黄腐酚和百里香酚对 影响龈下生物膜结构、大小和细胞活力的影响。通过扫描电子显微镜 (SEM) 和共聚焦激光显微镜 (CLSM) 比较了在未处理和两种提取物处理条件下培养 72 小时后生物膜的结构和微生物生物量。通过定量聚合酶链反应 (qPCR) 定量每种生物膜形成菌株在每种条件下的活菌和总微生物数量。使用一般线性模型比较和验证 CLSM 和 qPCR 结果。在生物膜形成过程中存在黄腐酚和百里香酚抑制 的丝状生长。与未暴露于提取物的生物膜相比，用黄腐酚孵育的生物膜的细菌生物量和细胞活力显著降低（<0.05）。相比之下，当生物膜用百里香酚孵育时，这些全局参数没有差异。在黄腐酚存在的情况下， 、 和 的计数和细胞活力降低。百里香酚处理降低了 和 的活力。在动态体外多物种生物膜模型的发展过程中存在这些植物提取物抑制了 的丝状生长，部分逆转了真菌对牙周病原菌结构、大小和活力的影响。
