ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) Research Group, Faculty of Dentistry, Complutense University, 28040 Madrid, Spain.
Department of Periodontology, Faculty of Dentistry, University of Porto, 4200-393 Porto, Portugal.
Int J Mol Sci. 2023 Jan 25;24(3):2335. doi: 10.3390/ijms24032335.
In search for natural products with antimicrobial properties for use in the prevention and treatment of peri-implantitis, the purpose of this investigation was to evaluate the antimicrobial activity of curcumin and xanthohumol, using an in vitro multi-species dynamic biofilm model including and . The antimicrobial activities of curcumin (5 mM) and xanthohumol (100 μM) extracts, and the respective controls, were evaluated with 72-h biofilms formed over dental implants by their submersion for 60 seconds. The evaluation was assessed by quantitative polymerase chain reaction (qPCR), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). For the data analysis, comparisons were tested applying ANOVA tests with Bonferroni corrections to evaluate the antimicrobial activity of both extracts. With qPCR, statistically significant reductions in bacterial counts were observed for curcumin and xanthohumol, when compared to the negative control. The results with CLSM and SEM were consistent with those reported with qPCR. It was concluded that both curcumin and xanthohumol have demonstrated antimicrobial activity against the six bacterial species included in the dynamic in vitro biofilm model used.
为了寻找具有抗菌特性的天然产物，用于预防和治疗种植体周围炎，本研究旨在使用包括 和 的体外多物种动态生物膜模型评估姜黄素和黄腐酚的抗菌活性。将姜黄素（5 mM）和黄腐酚（100 μM）提取物及其相应的对照物分别浸泡 60 秒，评估其对牙种植体上形成的 72 小时生物膜的抗菌活性。通过定量聚合酶链反应（qPCR）、共聚焦激光扫描显微镜（CLSM）和扫描电子显微镜（SEM）进行评估。对于数据分析，应用方差分析（ANOVA）测试，并应用 Bonferroni 校正来测试两种提取物的抗菌活性。qPCR 结果表明，与阴性对照相比，姜黄素和黄腐酚对细菌计数的抑制作用具有统计学意义。CLSM 和 SEM 的结果与 qPCR 报道的结果一致。因此，姜黄素和黄腐酚均对体外动态生物膜模型中包含的六种细菌表现出抗菌活性。
