Birchler J A, Schwartz D
Biochem Genet. 1979 Dec;17(11-12):1173-80. doi: 10.1007/BF00504355.
The alcohol dehydrogenase-1 FCm (Adh-FCm) duplication in maize was subjected to ethyl methanesulfonate (EMS) mutagenesis. Of the mutants recovered, eight produced ADH polypeptides with altered electrophoretic mobility. Four produced new mobilities of the progenitor F with no change of the Cm molecule; the remainder altered only the Cm enzyme. No cases were found in which the electrophoretic mobilities of the two types of subunits were simultaneously altered, and no complete nulls lacking both F and Cm were recovered. These observations confirm the duplicate nature of the FCm complex.
对玉米中的乙醇脱氢酶 -1 FCm（Adh - FCm）重复序列进行了甲磺酸乙酯（EMS）诱变处理。在回收的突变体中，有八个产生了电泳迁移率改变的ADH多肽。四个产生了祖代F的新迁移率，而Cm分子没有变化；其余的只改变了Cm酶。未发现两种亚基的电泳迁移率同时改变的情况，也未回收同时缺乏F和Cm的完全无效突变体。这些观察结果证实了FCm复合物的重复性质。
