用于检测环境中诱变剂的玉米乙醇脱氢酶基因座
Adh locus in maize for detection of mutagens in the environment.
作者信息
Schwartz D
出版信息
Environ Health Perspect. 1981 Jan;37:75-7. doi: 10.1289/ehp.813775.
Abstract
The advantages of the maize Adh system for low level mutagenesis studies are discussed. They include high mutation rate and the availability of both a strong selection system for null mutants and a sensitive method for detection of variants. The null selection involves treatment of pollen with allyl alcohol which is converted by the Adh enzyme to the toxic acrylaldehyde. Adh variants can be detected by alteration in affinity for phytic acid as well as thermal stability and electrophoretic mobility.
摘要
本文讨论了玉米乙醇脱氢酶（Adh）系统在低水平诱变研究中的优势。这些优势包括高突变率，以及针对无效突变体的强大选择系统和检测变体的灵敏方法。无效选择涉及用烯丙醇处理花粉，烯丙醇会被Adh酶转化为有毒的丙烯醛。可以通过对植酸亲和力的改变、热稳定性和电泳迁移率来检测Adh变体。
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