Monell Chemical Senses Center, Philadelphia, PA 19104, USA.
Fox Chase Cancer Center, Temple University, Philadelphia, PA 19111, USA.
Int J Mol Sci. 2024 Nov 8;25(22):12020. doi: 10.3390/ijms252212020.
Current research strongly suggests that phenolic compounds in extra virgin olive oil (EVOO) are potent preventive and therapeutic agents against metabolic diseases associated with inflammation and oxidative stress. Oleocanthal (OC) and oleacein (OA) are two of the most abundant and promising EVOO phenolics. To fully establish their health-promoting efficacy, additional animal studies and human clinical trials must be conducted, but the sourcing of both compounds at gram scale, reasonable cost, and ease of access remains a challenge. Here, we describe an extraction procedure to obtain OC and OA from the common privet (), a fast-growing, semi-evergreen shrub. We show that, compared to the olive tree, in addition to its broader geographical distribution, offers the benefit of yielding both OA and OC from its leaves. We also demonstrate the necessity of providing adapted enzymatic conditions during leaf treatment to optimize OC and OA concentrations in the final extracts.
目前的研究强烈表明，特级初榨橄榄油 (EVOO) 中的酚类化合物是预防和治疗与炎症和氧化应激相关的代谢疾病的有效物质。油橄榄素 (OC) 和橄榄苦苷 (OA) 是 EVOO 中最丰富、最有前途的两种酚类化合物。为了充分证实它们的促进健康的功效，还必须进行更多的动物研究和人体临床试验，但以合理的成本和便捷的方式在克级规模上获得这两种化合物仍然是一个挑战。在这里，我们描述了一种从常见的女贞 (Ligustrum vulgare) 中提取 OC 和 OA 的方法，女贞是一种生长迅速、半常绿的灌木。我们表明，与橄榄树相比，女贞除了分布范围更广之外，还具有从其叶子中同时提取 OA 和 OC 的优势。我们还证明了在叶子处理过程中提供适应的酶条件的必要性，以优化最终提取物中 OC 和 OA 的浓度。
