Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1 02-097, Warsaw, Poland.
Department of Pharmacodynamics, Centre for Preclinical Research and Technology, Medical University of Warsaw 02-097, Warsaw, Poland.
Phytomedicine. 2018 Oct 1;49:75-82. doi: 10.1016/j.phymed.2018.06.006. Epub 2018 Jun 11.
Chronic hyperalgesia and allodynia associated with progressive damage of peripheral neurons are the most prevalent complications of diabetes mellitus. Plants belonging to the family of Oleaceae were traditionally used in folk medicine for the management of diabetes.
HYPOTHESIS/PURPOSE: The aim of this study was to investigate whether an aqueous extract from the leaves of Ligustrum vulgare (common privet) could be useful to target neuropathic pain in a rat streptozotocin (STZ) model of diabetes.
The chemical composition of the aqueous extract from privet leaf was characterized with the UHPLC-DAD-MS method and the analytical quantification of its constituents was performed with HPLC-DAD. Mechanical hyperalgesia and allodynia were evaluated with the Randall-Selitto and von Frey tests.
Our investigation revealed the presence of secoiridoids: oleacein (23.48 ± 0.87 mg/g), oleocanthal (8.44 ± 0.08 mg/g), oleuropein (1.50 ± 0.01 mg/g), as well as phenylpropanoids: echinacoside (6.46 ± 0.07 mg/g), verbascoside (4.03 ± 0.04 mg/g) and p-coumaroyl glucarates in the dried aqueous extract of privet leaves. Behavioral data indicated that chronic intraperitoneal administration of the extract (50-200 mg/kg) for 21 days resulted in a decrease in diabetes-induced hyperalgesia and allodynia. Blood glucose levels remained unaltered, while body weight and water intake decreased significantly.
The aqueous privet leaf extract could serve useful in facilitating treatment of painful diabetic neuropathy. Additionally, the study showed that the antihyperalgesic activity of Ligustrum vulgare leaf extract is not likely related to its antihyperglycemic properties.
与外周神经元进行性损伤相关的慢性痛觉过敏和感觉异常是糖尿病最常见的并发症。属于木犀科的植物在传统医学中被用于治疗糖尿病。
假设/目的：本研究旨在探讨叶女贞（普通女贞）的水提物是否可用于治疗糖尿病大鼠链脲佐菌素（STZ）模型中的神经病理性疼痛。
采用 UHPLC-DAD-MS 法对女贞叶水提物的化学成分进行了表征，并采用 HPLC-DAD 对其成分进行了分析定量。采用 Randall-Selitto 和 von Frey 试验评价机械性痛觉过敏和感觉异常。
我们的研究发现，该水提物中存在环烯醚萜类化合物：橄榄苦苷（23.48±0.87mg/g）、橄榄苦苷（8.44±0.08mg/g）、橄榄苦苷（1.50±0.01mg/g），以及苯丙素类化合物：毛蕊花糖苷（6.46±0.07mg/g）、毛蕊花糖苷（4.03±0.04mg/g）和 p-香豆酰葡萄糖苷在女贞叶干水提物中。行为数据表明，慢性腹腔内给予该提取物（50-200mg/kg）21 天可降低糖尿病引起的痛觉过敏和感觉异常。血糖水平保持不变，而体重和饮水量显著下降。
女贞叶水提物可用于治疗痛性糖尿病性周围神经病变。此外，研究表明，女贞叶提取物的抗痛觉过敏活性与其降血糖作用无关。
