College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Int J Mol Sci. 2024 Nov 17;25(22):12334. doi: 10.3390/ijms252212334.
WD40 proteins play important roles in the synthesis and regulation of anthocyanin, the regulation of plant morphology and development, and the response to various abiotic stresses. However, the role of WD40 in () has not been studied. In this study, a total of 216 FvWD40 family members were identified, which were divided into four subfamilies based on evolutionary tree analysis. Subcellular localization predictions show that FvWD40 family members are mainly localized in chloroplasts, nuclei, and cytoplasm. An analysis of collinearity revealed a total of eight pairs of intraspecific collinearity of the gene family, and interspecific collinearity showed that the gene family covaried more gene pairs with () than with rice (). Promoter cis-acting elements revealed that the gene family contains predominantly light, hormone, and abiotic stress response elements. Tissue-specific expression analysis showed that a number of members including and were highly expressed in all tissues, and a number or members including and were lowly expressed in all tissues. The gene family was found to be expressed at all four different coloring stages of by qRT-PCR, with lower expression at the 50% coloring stage (S3). , , and showed the highest expression during the white fruit stage (S1) period, suggesting that these genes play a potential regulatory role in the pre-fruit coloring stage. , and had the highest expression at the 20% coloration stage (S2), and , , and had the highest expression at the full coloration stage (S4). These results suggest a potential role for these genes during fruit coloration. This study lays a foundation for further research on the function of the gene family.
WD40 蛋白在花色苷的合成和调控、植物形态和发育的调控以及对各种非生物胁迫的响应中发挥着重要作用。然而，WD40 在（）中的作用尚未得到研究。在本研究中，共鉴定出 216 个 FvWD40 家族成员，根据进化树分析将其分为四个亚家族。亚细胞定位预测表明，FvWD40 家族成员主要定位于叶绿体、核和细胞质。共线性分析显示，该基因家族共存在 8 对种内共线性，种间共线性表明，该基因家族与（）的共变基因对多于与水稻的共变基因对。启动子顺式作用元件分析表明，该基因家族主要含有光、激素和非生物胁迫响应元件。组织特异性表达分析表明，包括和在内的许多成员在所有组织中均高度表达，而包括和在内的许多成员在所有组织中均低度表达。通过 qRT-PCR 发现该基因家族在（）的四个不同着色阶段均有表达，在 50%着色阶段（S3）表达水平较低。、和在白果期（S1）表达水平最高，表明这些基因在果实着色前阶段可能发挥潜在的调控作用。、和在 20%着色阶段（S2）表达水平最高，而、、和在全着色阶段（S4）表达水平最高。这些结果表明这些基因在果实着色过程中可能发挥作用。本研究为进一步研究该基因家族的功能奠定了基础。
