Calvo Maddalena, Lauricella Flavio, Mellini Anna Maurizia, Scalia Guido, Trovato Laura
Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, Italy.
U.O.C. Laboratory Analysis Unit, A.O.U. Policlinico "G. Rodolico-San Marco" Catania, 95123 Catania, Italy.
Antibiotics (Basel). 2024 Oct 25;13(11):1005. doi: 10.3390/antibiotics13111005.
Pulmonary aspergillosis is a severe respiratory infection caused by spp., whose resistance profiles and invasive attitude complicate therapeutical strategies. Several aspergillosis cases emerged as superinfections during the SARS-CoV-2 pandemic when isavuconazole and amphotericin B became essential antifungal alternatives. The main purpose of the present study was to investigate a possible synergic activity between these molecules against spp. isolated from respiratory samples. . The gradient test method detected isavuconazole and amphotericin B MIC values, prompting an arrangement of their combination into an R.P.M.I. agar medium. According to Liofilchem s.r.l. instructions, the FIC index was used to establish synergy, additivity, indifference, or antagonism. . Among 36 spp. isolates, only strains showed both synergy and additivity episodes. reported the highest antagonism percentage, while revealed several indifference episodes. . Isavuconazole and amphotericin B remain fundamental therapeutical alternatives, including a possible synergic effect against . On the basis of this species-related difference, further studies will be essential to investigate different antifungal drug combinations against filamentous fungi isolates.
肺曲霉病是由曲霉属物种引起的一种严重呼吸道感染，其耐药情况和侵袭性使治疗策略变得复杂。在严重急性呼吸综合征冠状病毒2（SARS-CoV-2）大流行期间，当艾沙康唑和两性霉素B成为重要的抗真菌替代药物时，出现了几例曲霉病合并感染病例。本研究的主要目的是调查这些分子对从呼吸道样本中分离出的曲霉属物种是否具有协同活性。梯度试验方法检测了艾沙康唑和两性霉素B的最低抑菌浓度（MIC）值，并促使将它们的组合配置到罗伊氏改良伊格尔培养基（R.P.M.I.）琼脂培养基中。根据利奥菲化学有限公司的说明，使用联合抑菌系数（FIC）指数来确定协同作用、相加作用、无相互作用或拮抗作用。在36株曲霉属物种分离株中，只有菌株表现出协同和相加作用情况。报告的拮抗作用百分比最高，而显示出几种无相互作用情况。艾沙康唑和两性霉素B仍然是重要的治疗选择，包括对曲霉属可能具有协同作用。基于这种物种相关差异，进一步研究对于调查针对丝状真菌分离株的不同抗真菌药物组合至关重要。
