Seo Yoseph, Woo Yeeun, Oh Byeolnim, Yoo Daehyeon, Kwon Hyeok Ki, Park Chulhwan, Cho Hyeon-Yeol, Kim Hyun Soo, Lee Taek
Department of Chemical Engineering, Kwangwoon University, 20 Kwangwoon-ro, Nowon-gu, Seoul 01897, Republic of Korea.
Department of Electronic Engineering, Kwangwoon University, 20 Kwangwoon-ro, Nowon-gu, Seoul 01897, Republic of Korea.
Materials (Basel). 2024 Nov 13;17(22):5550. doi: 10.3390/ma17225550.
Microfluid-derived liposomes (M-Lipo) exhibit great potential as drug and functional substance carriers in pharmaceutical and food science. However, the low liposome membrane stability, attributed to the liquid core, limits their application range. Oleosin, a natural surfactant protein, can improve the stability of the lipid nanoparticle membrane against various environmental stresses, such as heat, drying, and pH change; in addition, it can enable sustained drug release. Here, we proposed the fabrication of oleosin-coated M-Lipo (OM-Lipo) through self-assembly on a microfluidic chip and the evaluation of its anticancer drug (carmustine) delivery efficiency. Nanoparticle characterization revealed that the oleosin coating slightly lowered the membrane potential of M-Lipo and greatly improved their dispersibility. Additionally, the in vitro drug release profile showed that the oleosin coating improved the sustained release of the hydrophobic drug from the phospholipid bilayer in body temperature. Our results suggest that OM-Lipo has sufficient potential in various fields, based on its easy production, excellent stability, and biocompatibility.
微流控衍生脂质体（M-Lipo）在制药和食品科学领域作为药物和功能物质载体具有巨大潜力。然而，由于液芯导致的脂质体膜稳定性低，限制了它们的应用范围。油质蛋白是一种天然表面活性剂蛋白，可提高脂质纳米颗粒膜对各种环境压力（如热、干燥和pH变化）的稳定性；此外，它还能实现药物的持续释放。在此，我们提出通过在微流控芯片上自组装制备油质蛋白包被的M-Lipo（OM-Lipo），并评估其抗癌药物（卡莫司汀）的递送效率。纳米颗粒表征显示，油质蛋白包被略微降低了M-Lipo的膜电位，并大大提高了它们的分散性。此外，体外药物释放曲线表明，油质蛋白包被改善了疏水性药物在体温下从磷脂双层中的持续释放。我们的结果表明，基于其易于生产、出色的稳定性和生物相容性，OM-Lipo在各个领域具有足够的潜力。
