Cissé Abdoulaye, Sambe Ba Bissoume, Sow Ousmane, Wane Abdoul Aziz, Ndiaye Issa, Fall Cheikh, Camara Makhtar, Dieye Yakhya
Pôle de Microbiologie, Institut Pasteur de Dakar, Dakar BP 220, Senegal.
Laboratoire de Bactériologie-Virologie, Hôpital Aristide le Dantec, Dakar BP 3001, Senegal.
Microorganisms. 2024 Nov 19;12(11):2357. doi: 10.3390/microorganisms12112357.
Extended-spectrum β-lactamase-producing (ESBL-) poses a significant public health concern due to its widespread prevalence and resistance to multiple antibiotics. This study aimed to assess the prevalence, resistance profile, and carriage of ESBL-encoding genes in ESBL- isolates from broilers in two markets of Dakar, Senegal. Sampling over a one-year period revealed that 61.7% of broilers carried ESBL- in their cecum. Antibiotic susceptibility testing of 186 ESBL- isolates showed high resistance to β-lactam antibiotics, including ampicillin, ticarcillin, and third-generation cephalosporins. Notably, all isolates were susceptible to imipenem. Multidrug resistance was frequent, with 91.4% of the isolates displaying this phenotype. Comparison between the two markets revealed variations in resistance to quinolones. PCR analysis detected genes in all isolates, with being the most prevalent subgroup. Additionally, and genes were found in 26.3% and 2.7% of isolates, respectively, while no genes were detected. Combinations of ESBL genes were common, with being the most frequent. These findings highlight the widespread prevalence of ESBL- in broilers from Dakar markets, indicating a potential risk of transmission to humans through the food chain. Effective surveillance and intervention strategies are crucial to mitigate the spread of antimicrobial resistance in humans and animals.
产超广谱β-内酰胺酶（ESBL）因其广泛流行和对多种抗生素耐药而成为重大的公共卫生问题。本研究旨在评估塞内加尔达喀尔两个市场肉鸡ESBL分离株中ESBL编码基因的流行情况、耐药谱及携带情况。为期一年的采样显示，61.7%的肉鸡盲肠中携带ESBL。对186株ESBL分离株进行的抗生素敏感性测试表明，它们对包括氨苄西林、替卡西林和第三代头孢菌素在内的β-内酰胺类抗生素具有高度耐药性。值得注意的是，所有分离株对亚胺培南敏感。多重耐药很常见，91.4%的分离株表现出这种表型。两个市场之间的比较显示对喹诺酮类药物的耐药性存在差异。PCR分析在所有分离株中均检测到基因，其中是最常见的亚组。此外，分别在26.3%和2.7%的分离株中发现了和基因，而未检测到基因。ESBL基因组合很常见，其中最为频繁。这些发现突出了达喀尔市场肉鸡中ESBL的广泛流行，表明存在通过食物链传播给人类的潜在风险。有效的监测和干预策略对于减轻人类和动物中抗菌药物耐药性的传播至关重要。
