Veksler A M, Kublik L I, Eĭdus L Kh
Med Radiol (Mosk). 1986 Apr;31(4):59-62.
The effect of enhancement of reproductive death in postradiation reduction of intracellular pH(pHk) was studied on Chinese hamster hypoxic cells. It was shown that pHk reduction up to 6.0 for 1 h enhanced the radiation damaging effect. The maximum effect was observed in pHk reduction shortly after irradiation. The dependence of the effect on the interval between irradiation and subsequent pHk reduction was different under various conditions of postradiation incubation: in good oxygenation due to fast reparation of potential injuries the effect of pHk reduction sharply decreased with time; the effect of additional influence of pHk reduction was insignificant against a background of low survival of cells incubated after radiation under hypoxic conditions. It was assumed that low survival under postradiation hypoxic conditions was determined by pHk reduction shown in this study during cell incubation under hypoxic conditions.
研究了在受辐射后降低中国仓鼠缺氧细胞内pH值（pHk）时增强生殖死亡的效应。结果表明，pHk降低至6.0并持续1小时会增强辐射损伤效应。在辐照后不久降低pHk时观察到最大效应。在不同的辐射后孵育条件下，该效应对辐照与随后pHk降低之间间隔的依赖性有所不同：在良好的氧合状态下，由于潜在损伤的快速修复，pHk降低的效应随时间急剧下降；在缺氧条件下辐射后孵育的细胞低存活率背景下，降低pHk的额外影响作用不显著。据推测，辐射后缺氧条件下的低存活率是由本研究中缺氧条件下细胞孵育期间pHk降低所决定的。
