Laboratory of Organic Chemistry, Department of Chemical Sciences, School of Chemical Engineering, National Technical University of Athens, 15772 Athens, Greece.
Laboratory of Chemistry, Analysis and Design of Food Processes, Department of Food Science and Technology, University of West Attica, 12243 Athens, Greece.
Molecules. 2024 Nov 12;29(22):5318. doi: 10.3390/molecules29225318.
A hybrid alginate hydrogel-chitosan nanoparticle system suitable for biomedical applications was prepared. Chitosan (CS) was used as a matrix for the encapsulation of lavender () essential oil (LEO) and Mentha () essential oil (MEO). An aqueous solution of an acidic Natural Deep Eutectic Solvent (NADES), namely choline chloride/ascorbic acid in a 2:1 molar ratio, was used to achieve the acidic environment for the dissolution of chitosan and also played the role of the ionic gelator for the preparation of the chitosan nanoparticles (CS-NPs). The hydrodynamic diameter of the CS-MEO NPs was 130.7 nm, and the size of the CS-LEO NPs was 143.4 nm (as determined using Nanoparticle Tracking Analysis). The CS-NPs were incorporated into alginate hydrogels crosslinked with CaCl. The hydrogels showed significant water retention capacity (>80%) even after the swollen sample was kept in the aqueous HCl solution (pH 1.2) for 4 h, indicating a good stability of the network. The hydrogels were tested (a) for their ability to absorb dietary lipids and (b) for their antimicrobial activity against Gram-positive and Gram-negative foodborne pathogens. The antimicrobial activity of the hybrid hydrogels was comparable to that of the widely used food preservative sodium benzoate 5% /.
一种适用于生物医学应用的藻酸盐水凝胶-壳聚糖纳米粒子系统被制备。壳聚糖（CS）被用作薰衣草（）精油（LEO）和薄荷（）精油（MEO）包封的基质。一种酸性天然深共晶溶剂（NADES）的水溶液，即胆碱氯化物/抗坏血酸的摩尔比为 2:1，被用于实现壳聚糖的溶解的酸性环境，并且还起到壳聚糖纳米粒子（CS-NPs）的离子凝胶剂的作用。CS-MEO NPs 的水动力直径为 130.7nm，CS-LEO NPs 的大小为 143.4nm（使用纳米颗粒跟踪分析确定）。CS-NPs 被掺入用 CaCl 交联的藻酸盐水凝胶中。水凝胶表现出显著的保水能力（>80%），即使在膨胀样品在 pH 值为 1.2 的水性 HCl 溶液中保持 4 小时后，表明网络具有良好的稳定性。测试了水凝胶（a）吸收膳食脂质的能力和（b）对革兰氏阳性和革兰氏阴性食源性病原体的抗菌活性。混合水凝胶的抗菌活性可与广泛使用的食品防腐剂苯甲酸钠 5%相媲美/。
