Deng Linmei, Huang Xun, Dao Jian, Xu Yajin, Zhou Kunyan, Wang Wenping, Liu Chunjiang, Chen Meng, Zhang Shunhong, Zhang Yue, Hao Jianjun, Liu Xia, Yang Yanli
Key Laboratory for Agro-biodiversity and Pest Control of Ministry of Education, College of Plant Protection, Yunnan Agricultural University, Kunming, China.
School of Food and Agriculture, The University of Maine, Orono, ME, United States.
Front Plant Sci. 2024 Nov 12;15:1481165. doi: 10.3389/fpls.2024.1481165. eCollection 2024.
Late blight caused by is the most devastating disease of potato. produces many secondary metabolites and effector proteins, involved in the pathogenesis, which compromise host defense mechanisms. Pectinesterase (PE) is a cell wall degrading enzyme secreted by to infect the host. To examine the role of PE in , 15 strains of were isolated from infected potato leaves in Yunnan, China. We analyzed the biological effects of exogenously added PE on and its activity and gene expression after infection of potato using quantitative real-time polymerase chain reaction (RT-PCR). It was found that PE significantly promotes the growth of , increases the weight of mycelium and the number of sporangia, and promotes the sporangial germination. PE accelerated the infection process of on potato. The pathogenicity of was positively correlated with PE activity and gene expression. PE is a key to the virulence difference of potato late blight.
由[病原菌名称缺失]引起的晚疫病是马铃薯最具毁灭性的病害。[病原菌名称缺失]产生许多参与致病过程的次生代谢产物和效应蛋白，这些会破坏宿主的防御机制。果胶酯酶（PE）是[病原菌名称缺失]分泌的一种细胞壁降解酶，用于感染宿主。为了研究PE在[病原菌名称缺失]致病中的作用，从中国云南感染晚疫病的马铃薯叶片中分离出15株[病原菌名称缺失]菌株。我们使用定量实时聚合酶链反应（RT-PCR）分析了外源添加的PE对[病原菌名称缺失]的生物学效应及其在感染马铃薯后的活性和基因表达。结果发现，PE显著促进[病原菌名称缺失]的生长，增加菌丝体重量和孢子囊数量，并促进孢子囊萌发。PE加速了[病原菌名称缺失]对马铃薯的感染过程。[病原菌名称缺失]的致病性与PE活性和基因表达呈正相关。PE是马铃薯晚疫病菌毒力差异的关键因素。
