Deng Linmei, Hao Jianjun, Feng Jiawen, Zhao Jing, Dao Jian, Xu Gaihuan, Zhou Kunyan, Xu Yajin, Wang Wenping, Zhang Shunhong, Liu Chunjiang, Chen Meng, Yang Yanli, Liu Xia
Key Laboratory for Agro-biodiversity and Pest Control of Ministry of Education, College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, China.
School of Food and Agriculture, The University of Maine, Orono, 04469, ME, U.S.A.
BMC Plant Biol. 2025 May 28;25(1):716. doi: 10.1186/s12870-025-06736-y.
Phytophthora infestans causes potato late blight and significantly impacts potato production. The pathogen's remarkable adaptability and ability to generate new physiological races through virulence variation undermine varietal resistance, posing challenges for disease prevention and control. To explore the genetic mechanisms behind these, different physiological races of P. infestans were inoculated on potato leaves and assayed through transcriptomics combined with metabolic profiling methods. It was found that the DL04 strain, which carries virulence factor 3, exhibited a high level of pathogenicity. Biologically, DL04 showed more rapid growth and denser mycelial structures compared to most other strains, indicating enhanced pathogenicity. DL04 induced a greater enrichment of carbon metabolism, biosynthesis of amino acids, and glycolysis/gluconeogenesis pathways during the infection process. This led to the upregulation of genes related to cell hydrolysis, accelerating leaf infection and contributing to its higher level of pathogenicity. The reasons for the differences in pathogenicity among different physiological races of P. infestans were clarified at the transcriptional level. This finding provides valuable insights into the genetic basis of P. infestans pathogenicity and offer critical information for developing effective control strategies, breeding for disease resistance, and improving potato production practice.
致病疫霉引发马铃薯晚疫病，对马铃薯生产造成重大影响。该病原菌具有显著的适应性，能够通过毒性变异产生新的生理小种，从而削弱品种抗性，给病害防控带来挑战。为探究其背后的遗传机制，将致病疫霉的不同生理小种接种到马铃薯叶片上，并结合代谢组学方法通过转录组学进行分析。结果发现，携带毒性因子3的DL04菌株具有高度致病性。从生物学角度来看，与大多数其他菌株相比，DL04生长更快，菌丝结构更密集，表明其致病性增强。在感染过程中，DL04诱导碳代谢、氨基酸生物合成以及糖酵解/糖异生途径出现更大程度的富集。这导致与细胞水解相关的基因上调，加速叶片感染，并使其致病性更高。在转录水平上阐明了致病疫霉不同生理小种致病性差异的原因。这一发现为致病疫霉致病性的遗传基础提供了有价值的见解，并为制定有效的防控策略、抗病育种以及改进马铃薯生产实践提供了关键信息。
