Shang Kaijie, Wang Chenchen, Wang Xipan, Wang Yubo, Xu Kaihao, Zhou Shumei, Liu Hongmei, Zhu Xiaoping, Zhu Changxiang
College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, China.
Plant Cell Environ. 2025 Mar;48(3):2343-2356. doi: 10.1111/pce.15298. Epub 2024 Nov 27.
Plant viruses rely on host factors for successful infection. Non-specific lipid transfer proteins (nsLTPs) play critical roles in plant-pathogen interactions; however, their functions and underlying molecular mechanisms in viral infections remain largely unknown. Jasmonic acid (JA) is a crucial regulatory hormone in the process of plant resistance to viral infection. In this study, we screened and verified that StLTP6, a previously identified pro-viral factor, interacts with the silencing suppressor triple gene block1 (TGB1) of potato virus S (PVS). The PVS TGB1 induces the expression of StLTP6, and both co-localize in the cytoplasm. Furthermore, StLTP6 interacts with allene oxide cyclase and inhibits its accumulation, thereby suppressing JA synthesis and attenuating RNA silencing antiviral resistance. In summary, we elucidated the molecular mechanism by which PVS TGB1 interacts with StLTP6 to facilitate PVS infection. These findings broaden our understanding of the biological roles of nsLTPs and provide a new antiviral target for potato research.
植物病毒依靠宿主因子来成功感染。非特异性脂质转移蛋白（nsLTPs）在植物与病原体的相互作用中起关键作用；然而，它们在病毒感染中的功能及潜在分子机制在很大程度上仍不清楚。茉莉酸（JA）是植物抗病毒感染过程中的一种关键调节激素。在本研究中，我们筛选并验证了先前鉴定的促病毒因子StLTP6与马铃薯S病毒（PVS）的沉默抑制子三基因块1（TGB1）相互作用。PVS TGB1诱导StLTP6的表达，且二者共定位于细胞质中。此外，StLTP6与丙二烯氧化物环化酶相互作用并抑制其积累，从而抑制JA合成并减弱RNA沉默抗病毒抗性。总之，我们阐明了PVS TGB1与StLTP6相互作用以促进PVS感染的分子机制。这些发现拓宽了我们对nsLTPs生物学作用的理解，并为马铃薯研究提供了一个新的抗病毒靶点。
