Lukács L, István M, Tóth P
Neoplasma. 1986;33(1):11-9.
The authors investigated the lymphonodal response to peripherally administered 2,4-dinitrochlorobenzene (DNCB). DNCB is known as a strong irritant binding to membrane proteins of the Langerhans cells of the skin. The conjugate is a potent neoantigen able to induce local tumor-destruction and raise cellular immune defence. White laboratory rats were used for the experiments. Their popliteal lymph nodes were investigated after sensitization with DNCB and i.c. injection of H3-thymidine and Patent Blue Violet at several intervals following the challenge with the hapten DNCB. Changes of weight of lymph nodes further uptake of H3-thymidine by the cells were investigated by autoradiography, while the content of H3-DNA indicating an increased DNA-metabolism due to blastic transformation of the small lymphocytes, was determined quantitatively by a liquid scintillation method. The data obtained speak for the usefulness of DNCB as an immunostimulant, comparable to BCG but of less toxicity.
作者研究了外周给予2,4-二硝基氯苯（DNCB）后淋巴结的反应。DNCB是一种已知的强刺激物，可与皮肤朗格汉斯细胞的膜蛋白结合。该结合物是一种有效的新抗原，能够诱导局部肿瘤破坏并增强细胞免疫防御。实验使用了白色实验大鼠。在用DNCB致敏并在半抗原DNCB激发后的几个时间间隔进行腹腔注射H3-胸腺嘧啶核苷和专利蓝紫后，对其腘窝淋巴结进行了研究。通过放射自显影研究淋巴结重量的变化以及细胞对H3-胸腺嘧啶核苷的进一步摄取，而通过液体闪烁法对表明由于小淋巴细胞的母细胞转化导致DNA代谢增加的H3-DNA含量进行定量测定。所获得的数据表明DNCB作为一种免疫刺激剂是有用的，其效果与卡介苗相当，但毒性较小。
