Li Jihao, Bian Shuaishuai, Liu Tian, Li Hongxue, Li Jian, Ren Haohui, Zhang Wenjun, Lee Chun-Sing, Zheng Xiuli, Liu Weimin, Wang Pengfei
Key Laboratory of Photochemical Conversion and Optoelectronic Materials and CityU-CAS Joint Laboratory of Functional Materials and Devices, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China; School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100049, China.
Center of Super-Diamond and Advanced Films (COSDAF) & Department of Materials Science and Engineering, City University of Hong Kong, Hong Kong SAR, 999077, China.
Biosens Bioelectron. 2025 Feb 15;270:116978. doi: 10.1016/j.bios.2024.116978. Epub 2024 Nov 20.
The incorporation a "singlet oxygen (O) battery" into photodynamic therapy (PDT) could overcome the deficiency of tumor hypoxia in PDT and enhance its effect. However, real-time monitoring the O release efficiency of the O battery still presents a significant challenge in vivo. To address this issue, we have developed a bright aggregation-induced emission (AIE) chemiluminescence (CL) probe (DTLum), which conjugates a luminol unit with a donor-acceptor structured diketopyrrolopyrrole fluorophore, for the specific detection of O. Subsequently, the DTLum nanoparticles (DTLum NPs) were prepared using PEO-PPO-PEO (Pluronic F127) as the surfactant. The DTLum NPs can detect O in aqueous solution with a bright near-infrared (NIR) CL signal (651 nm) and great tissue penetration (12.5 mm), making them suitable for the detection of O both in vitro (quantitative) and in vivo (qualitative). Notably, by utilizing the DTLum NPs, the process of O release in O batteries with different release rates can be visually monitored in cells and in vivo. This NIR CL probe provides a powerful platform for real-time monitoring and evaluating the release efficiency of O battery.
将“单线态氧（O）电池”整合到光动力疗法（PDT）中可以克服PDT中肿瘤缺氧的不足并增强其效果。然而，在体内实时监测O电池的O释放效率仍然是一项重大挑战。为了解决这个问题，我们开发了一种明亮的聚集诱导发光（AIE）化学发光（CL）探针（DTLum），它将鲁米诺单元与供体-受体结构的二酮吡咯并吡咯荧光团结合，用于特异性检测O。随后，使用聚环氧乙烷-聚环氧丙烷-聚环氧乙烷（泊洛沙姆F127）作为表面活性剂制备了DTLum纳米颗粒（DTLum NPs）。DTLum NPs可以通过明亮的近红外（NIR）CL信号（651 nm）和良好的组织穿透性（12.5 mm）检测水溶液中的O，使其适用于体外（定量）和体内（定性）的O检测。值得注意的是，通过使用DTLum NPs，可以在细胞和体内直观地监测不同释放速率的O电池中O的释放过程。这种近红外CL探针为实时监测和评估O电池的释放效率提供了一个强大的平台。
