College of Chemical Engineering, Fuzhou University, Fuzhou, 350108, P. R. China.
College of Materials Science and Engineering, Fuzhou University, Fuzhou, 350108, P. R. China.
Small. 2022 Oct;18(41):e2202551. doi: 10.1002/smll.202202551. Epub 2022 Sep 11.
The development of probes for early monitoring tumor therapy response may greatly benefit the promotion of photodynamic therapy (PDT) efficacy. Singlet oxygen ( O ) generation is a typical indicator for evaluating PDT efficacy in cancer. However, most existing probes cannot quantitatively detect O in vivo due to the high reactivity and transient state, and thus have a poor correlation with PDT response. Herein, a O -responsive theranostic platform comprising thiophene-based small molecule (2SeFT-PEG) and photosensitizer Chlorin e6 (Ce6) micelles for real-time monitoring PDT efficacy is developed. After laser irradiation, the Ce6-produced O could simultaneously kill cancer and trigger 2SeFT-PEG to produce increased chemiluminescence (CL) and decreased fluorescence (FL) signals variation at 1050 nm in the second near-infrared (NIR-II, 950-1700 nm) window. Significantly, the ratiometric NIR-II CL/FL imaging at 1050 nm could effectively quantify and monitor the concentration of O and O consumption or recovery, so as to evaluate the therapeutic efficacy of PDT in vivo. Hence, this O activated NIR-II CL/FL probe provides an efficient ratiometric optical imaging platform for real-time evaluating PDT effect and precisely guiding the PDT process in vivo.
用于早期监测肿瘤治疗反应的探针的发展可能极大地有益于促进光动力疗法（PDT）的疗效。单线态氧（1O2）的产生是评估癌症中 PDT 疗效的典型指标。然而，由于高反应性和瞬态，大多数现有探针无法在体内定量检测 1O2，因此与 PDT 反应相关性较差。在此，开发了一种包含噻吩基小分子（2SeFT-PEG）和光敏剂氯（Ce6）胶束的 1O2 响应治疗平台，用于实时监测 PDT 疗效。激光照射后，Ce6 产生的 1O2 可以同时杀死癌细胞，并触发 2SeFT-PEG 在第二个近红外（NIR-II，950-1700nm）窗口中产生增加的化学发光（CL）和减少的荧光（FL）信号变化在 1050nm 处。重要的是，在 1050nm 处的比率 NIR-II CL/FL 成像可以有效地定量和监测 1O2 的浓度和 1O2 的消耗或恢复，从而评估体内 PDT 的治疗效果。因此，这种 1O2 激活的 NIR-II CL/FL 探针为实时评估 PDT 效果和精确指导体内 PDT 过程提供了一种有效的比率光学成像平台。
