Yang Mingwang, Zhang Junwei, Shabat Doron, Fan Jiangli, Peng Xiaojun
State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian 116024, P.R. China.
School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv 69978 Israel.
ACS Sens. 2020 Oct 23;5(10):3158-3164. doi: 10.1021/acssensors.0c01291. Epub 2020 Sep 28.
Singlet oxygen (O) plays a vital role in metabolism. However, because of its extremely high reactivity and short-lived state, the detection of O is challenging. To address this issue, for the first time, we herein constructed a near-infrared (NIR) chemiluminescent probe () by caging the precursor of phenoxy-dioxetane scaffolds and a dicyanomethylchromone acceptor for selective O detection. This probe can detect O with a tremendous turn-on chemiluminescence signal in the NIR region (700 nm) and image intracellular O produced by the photosensitizer during the simulated action of photodynamic therapy (PDT). Notably, O level changes in the abdominal cavity and tumor of the various mice model under different stimulations and PDT action were effectively monitored by , providing a novel chemiluminescence imaging platform to explore O generation in PDT-associated applications.
单线态氧（O）在新陈代谢中起着至关重要的作用。然而，由于其极高的反应活性和短寿命状态，对O的检测具有挑战性。为了解决这个问题，我们首次在此构建了一种近红外（NIR）化学发光探针（），通过将苯氧基二氧杂环丁烷支架的前体与二氰基甲基色酮受体结合，用于选择性检测O。该探针可以在近红外区域（700 nm）以巨大的开启化学发光信号检测O，并对光动力疗法（PDT）模拟作用期间光敏剂产生的细胞内O进行成像。值得注意的是，通过该探针有效地监测了不同刺激和PDT作用下各种小鼠模型腹腔和肿瘤中O水平的变化，为探索PDT相关应用中O的产生提供了一个新型的化学发光成像平台。
