Neitz A W, Viljoen G J, Bezuidenhout J D, Oberem P T, van Wyngaardt W, Vermeulen N M
Onderstepoort J Vet Res. 1986 Mar;53(1):39-41.
A sensitive and reliable enzyme-linked immunosorbent assay for the detection of antibodies to Cowdria ruminantium in serum and C. ruminantium antigen in Amblyomma hebraeum nymphae is described. For the screening of antibodies, C. ruminantium from A. hebraeum nymphae, partially purified by wheat-germ lectin affinity chromatography, was used as antigen. To screen nymph populations, sera from either Ball 3 strain-infected sheep or Kumm-strain infected mice were used. By using appropriate controls the assays were rendered specific with respect to C. ruminantium.
本文描述了一种灵敏且可靠的酶联免疫吸附测定法，用于检测血清中反刍兽考德里氏体抗体以及希伯来花蜱若虫体内的反刍兽考德里氏体抗原。在抗体筛查中，使用经麦胚凝集素亲和层析部分纯化的希伯来花蜱若虫体内的反刍兽考德里氏体作为抗原。为筛查若虫群体，使用来自感染了Ball 3株的绵羊或感染了Kumm株的小鼠的血清。通过使用适当的对照，使该测定法对反刍兽考德里氏体具有特异性。
